Serological and molecular diagnosis of toxoplasmosis in pregnant women in Omdurman, Khartoum State

Abstract EN

This study was conducted to determine the sero-diagnosis and risk factors of toxoplasmaosis among pregnant women in Omdurman, Khartoume state, during the period of June 2006 to July 2008.

The study included 455 pregnant women between 25-35 years of ages.

The blood samples were collected and examined by direct latex agglutination, ELISA IgG and IgM and ten by PCR test.

All serological investigations were carried out in the center for research and laboratory sciences, Faculty of Medicine, Ain Shams University, Egypt.

Data were collected from the pregnant women by interview using modified questionnaire.

The results showed that the prevalence of Toxoplasma gondii was 17.

8 % by direct latex agglutination, 38.9% by ELISA IgG (indicating chronic infections) and 12.

9 % by IgMindicating acute infections.

The results of PCR test showed 16.

7 %.

Although of questionable accuracy, the results of the present study revealed a relatively high sero-prevalence of acute infection in the pregnant women, with Toxoplasma