Sequence analysis and preparation of DNA vaccine for equine influenza virus (H3N8)‎ isolated in Egypt 2008

Other Title(s)

تحليل التتابع التيوكليوتيدى و تحضير لقاح جيني لفيروس إنفلونزا الخيول 2008 عام مصر في المعزول (H3N8)‎

Dissertant

Ahmad, Basim Muhammad Abd al-Hamid

Thesis advisor

al-Sanusi, Ahmad Abd al-Ghani
Ahmad, Husayn Ali Husayn

University

Cairo University

Faculty

Faculty of Veterinary Medicine

University Country

Egypt

Degree

Master

Degree Date

2012

English Abstract

HAI part of the H3 gene of EIV was cloned in a mammalian expression vector the recombinant protein was de novo synthesized both in vitro and in vivo and assayed for expression and immunogenicity using FAT , HI and serum neutralization test , EIV which was isolated in previous study (Hussein et al.

, 2011) was propagated and detected using FLUDETECT ? ? test strip and titrated using HA and egg infectivity titration then identified by two sequential RT - PCR the first targeting internal part of EIV matrix gene while the second is targeting internal identifying part of EIV H3 hemagglutinin which was sequenced and analyzed computationally and found to be related to florida sublineage clade 1 EI viruses in another study (Ahmed et al .

, 2011

Main Subjects

Veterinary Medicine

Topics

• Diseases
• Egypt

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-576528