Molecular cloning and expression analysis of RrNHX1 and RrVHA-c genes related to salt tolerance in wild Rosa rugosa
Joint Authors
Wang, Jia
Feng, Liguo
Zang, Shu
Sheng, Lixia
Wang, Meng
Xia, Wei
Ding, Han
Source
Saudi Journal of Biological Sciences
Issue
Vol. 22, Issue 4 (31 Dec. 2015), pp.417-423, 7 p.
Publisher
Publication Date
2015-12-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Salt stress is one important factor influencing the growth and development of plants, and salt tolerance of plants is a result of combined action of multiple genes and mechanisms.
Rosa rugosa is not only an important ornamental plant, but also the natural aromatic plant of high value.
Wild R.
rugosa which is naturally distributed on the coast and islands of China has a good salt tolerance due to the special living environment.
Here, the vacuolar Na+/H+ reverse transporter gene (NHX1) and the vacuolar H+-ATPase subunit C gene (VHA-c) closely related to plant salt tolerance were isolated from wild R.
rugosa, and the expression patterns in R.
rugosa leaves of the two genes under NaCl stress were determined by real-time quantitative fluorescence PCR.
The results showed that the RrNHX1 protein is a constitutive Na+/H+ reverse transporter, the expression of the RrNHX1 gene first increased and then decreased with the increasing salt concentration, and had a time-controlled effect.
The RrVHA-c gene is suggestive of the housekeeping feature, its expression pattern showed a similar variation trend with the RrNHX1 gene under the stress of different concentrations of NaCl, and its temporal expression level under 200 mM NaCl stress presented bimodal change.
These findings indicated that RrNHX1 and RrVHA-c genes are closely associated with the salt tolerance trait of wild R.
rugosa.
ª 2015 The Authors.
Production and hosting by Elsevier B.V.
on behalf of King Saud University.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).-
American Psychological Association (APA)
Feng, Liguo& Ding, Han& Wang, Jia& Wang, Meng& Xia, Wei& Zang, Shu…[et al.]. 2015. Molecular cloning and expression analysis of RrNHX1 and RrVHA-c genes related to salt tolerance in wild Rosa rugosa. Saudi Journal of Biological Sciences،Vol. 22, no. 4, pp.417-423.
https://search.emarefa.net/detail/BIM-577369
Modern Language Association (MLA)
Feng, Liguo…[et al.]. Molecular cloning and expression analysis of RrNHX1 and RrVHA-c genes related to salt tolerance in wild Rosa rugosa. Saudi Journal of Biological Sciences Vol. 22, no. 4 (2015), pp.417-423.
https://search.emarefa.net/detail/BIM-577369
American Medical Association (AMA)
Feng, Liguo& Ding, Han& Wang, Jia& Wang, Meng& Xia, Wei& Zang, Shu…[et al.]. Molecular cloning and expression analysis of RrNHX1 and RrVHA-c genes related to salt tolerance in wild Rosa rugosa. Saudi Journal of Biological Sciences. 2015. Vol. 22, no. 4, pp.417-423.
https://search.emarefa.net/detail/BIM-577369
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 422-423
Record ID
BIM-577369