Improvement of halophilic cellulase production from locally isolated fungal strain
Joint Authors
Ghani, Ahmad Anas Nagoor
Arbain, Dachyar
Jamal, Parveen
Gumba, Rizo Edwin
Source
Saudi Journal of Biological Sciences
Issue
Vol. 22, Issue 4 (31 Dec. 2015), pp.476-483, 8 p.
Publisher
Publication Date
2015-12-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Abstract EN
Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses.
Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–Burman design and the Face Centered Central Composite Design (FCCCD).
Plackett–Burman experimental design was used to screen the medium components and process conditions.
It was found that carboxymethylcellulose (CMC), FeSO4Æ7H2O, NaCl, MgSO4Æ7H2O, peptone, agitation speed and inoculum size significantly influence the production of halophilic cellulase.
On the other hand, KH2PO4, KOH, yeast extract and temperature had a negative effect on enzyme production.
Further optimization through FCCCD revealed that the optimization approach improved halophilic cellulase production from 0.029 U/ml to 0.0625 U/ml, which was approximately 2.2-times greater than before optimization.
ª 2014 Production and hosting by Elsevier B.V.
on behalf of King Saud University.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
American Psychological Association (APA)
Ghani, Ahmad Anas Nagoor& Arbain, Dachyar& Jamal, Parveen& Gumba, Rizo Edwin. 2015. Improvement of halophilic cellulase production from locally isolated fungal strain. Saudi Journal of Biological Sciences،Vol. 22, no. 4, pp.476-483.
https://search.emarefa.net/detail/BIM-577520
Modern Language Association (MLA)
Ghani, Ahmad Anas Nagoor…[et al.]. Improvement of halophilic cellulase production from locally isolated fungal strain. Saudi Journal of Biological Sciences Vol. 22, no. 4 (2015), pp.476-483.
https://search.emarefa.net/detail/BIM-577520
American Medical Association (AMA)
Ghani, Ahmad Anas Nagoor& Arbain, Dachyar& Jamal, Parveen& Gumba, Rizo Edwin. Improvement of halophilic cellulase production from locally isolated fungal strain. Saudi Journal of Biological Sciences. 2015. Vol. 22, no. 4, pp.476-483.
https://search.emarefa.net/detail/BIM-577520
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 483
Record ID
BIM-577520