Flow injection spectrophotometric determination of thymol using 4-aminoantipyrine and copper(ii)‎ nitrate

Abstract EN

Flow-injection (FI) spectrophotometric method has been developed for the analysis of thymol in pharmaceutical preparations.

The method is based on organic coupling reaction between thymol and 4-amino antipyrine in the presence of alkaline medium to form an intense stable red color complex with copper nitrate that has a maximum absorption at 490 nm.

Optimum conditions for determination of the drug was investigated .The calibration graph was linear over the range of 5-500 μg.ml-1 of thymol .

The limit of detection (LOD) and limit of quantification (LOQ) were 1.81 μg mL-1 and 3.60 μg mL-1 respectively .The proposed method was applied satisfactorily to the determination of thymol in mouth wash preparations.

The procedure is characterized by its simplicity, accuracy and precision.