Immunohsitochemical study of CA19-9 overexpression in colorectal adenocarcinoma and its correlation with some pathological parameters

Abstract EN

Background : Colorectal carcinoma is third most frequent cancer in the world in both sexes and the third most frequent cause of cancer related deaths .Its incidence and mortality rates vary markedly around the world.

The risk of developing colorectal carcinoma increasing with age.

Most cases occur in the sixty and seventy years old , while cases before age fifty years old are uncommon unless there is a family history of early colon cancer.

The pathogensis of colorectal carcinoma is complex and developed in multisteps process in which several gene mutations will occur an coordinate with each other in genotyping and phenotyping outcome.

Aim of the study : To assess CA19-9 over expression in colorectal adenocarcinoma and its correlation to some of pathological parameters.

Materials and methods : this study was included fourty cases of colorectal carcinoma, collected randomly from the period of February 2010- May 2012 from Al Hilla Teaching Hospital.40 patients (30male and 10 females) of colorectal adenocarcinoma ,their median age 50 years range from (30 – 70) years.

A group of 10 patients with nonspecific colitis were included as a control group.

A manual avidin biotin peroxidase complex procedure(ABC) system was used in the imunohistochemical analysis (Dako Cytomation Copenhagen ,Denmark).

Results : CA19-9 over expression was positive in (70 %) of colorectal adenocarcinoma, while there was no expression in benign colonic lesions.

CA19-9 immunohistochemical staining was positively correlated with grade and stage of colorectal adenocarcinoma.

Conclusion : These finding support the role of CA19-9 in carcinogenesis of colorectal adenocarcinoma regarding behavior and aggressiveness, and thus CA19-9 could be considered as a poor prognostic parameter in colorectal adenocarcinoma