Spectrophotometric assay of metoclopramide hydrochloride in bulk and in dosage form

Abstract EN

A simple, reproducible and sensitive spectrophotometric method for assay of metoclopramide hydrochloride is investigated.

The method is based on the Schiff's base formation reaction of metoclopramide with 1,2-naphoquinone sulphonate (NQS) as chromogenic reagent in the presence of micellar cetylperidinium chloride (CPC) and sodium carbonate in aqueous solution to give a highly coloured product with maximum absorption at 471 nm.

Beer's law is obeyed in the range of 0.

1-26 g / ml with a molar absorptivity of 4.124 × 104 l.

mol-1.cm-1 and the limit of detection and quantitation are 0.

0416 and 0.1386 g / ml respectively.

The accuracy (average recovery %) is 100.

72 % and the relative standard deviation (RSD) is better than 0.

44 %.

Also, it was found that the product formed was in ratio of 1 : 1 product with stability constant of 3.

2 × 107 L.mol-1.

The method is applied successfully to the assay of metoclopramide hydrochloride in pharmaceutical formulations and is agreed well with its certified value and with those of British pharmacopoeia method.