Fabrication of novel collagenous dual-layer scaffolds

Abstract EN

Currently no generally simple applicable methodology is available for designing dual-layer cellular constructs in vitro that resemble the stratified, lattice-like structure in tissues mimicking the structure of tissues in vivo.

Novel processing techniques have been developed to manufacture reproducibly dual-layer collagen scaffolds and Hybrid Synthetic / Natural Scaffolds, with excellent adhesion and high porosities for cell seeding and large surface areas for cell attachment.

These scaffolds have been used to demonstrate the feasibility of regenerating several organs.

These constructs comprise of the lay down of lyophilized prefabricated collagen scaffold at –20 0C -80 0C or –196 0C on a second layer of collagen suspension of 0.8 % type I collagen or vice versa, subsequently the whole composite frozen at –20 0C, -80 0C or -196 0C, then lyophilized.

For the preparation of these scaffolds we relied on simple techniques without the need for expensive/customized equipment, hence allowing a methodology that can be used in every laboratory.