Protective effects of Punica granatum seed extract against ifosfamide induced sperm abnormalities in male albino mice

Abstract EN

The aim of the present study is to assess the ability of Punica granatum seed extract to cause and prevent the sperm shape abnormalities induced by the alkylating agent ifosfamide (IFO) in laboratory male albino mice Mus musculus of BALB/c strain.

The pomegranate seed extract (tablets) was provided by Source naturals, INC.

Santa Cruse Company, Canada (from Egypt).

In the present work, two independent experiments were performed.

In the first, mice was treated daily and orally with phosphate buffer saline (PBS) as negative control group, and the other three groups were treated daily and orally with three different doses of the Punica granatum seed extract (12.5, 25, and 50 mg / kg b.w.) for five weeks in order to test the ability of the plant seed extract to cause sperm abnormalities.

In the second experiment, the first group was positive control group which weekly and intraperitonially treated with ifosfamide (100 mg / kg b.w.), the other three groups were treated daily and orally with three different doses of the pomegranate seed extract (12.5, 25, and 50 mg / kg b.w.) and also intraperitonially treated at the end of each week with IFO for five weeks to test the protective effects Punica granatum seed extract.

The results of the first experiment are indicated that the Punica granatum seed extract has not negative significant effects on sperm abnormalities of treated mice.

The results of the second cleared that the Punica granatum seed extract, especially at the third dose (50 mg/kg b.w.) exhibited well protective effect against the damage actions of the ifosfamide on sperm shape.