Identification of edwardsiella tarda isolated from tilapia nilotica (oreochromis niloticus) by polymerase chain reaction (PCR). the effect of garlic oil and different antimicrobials on the identified organism in vitro
Other Title(s)
التعرف على الأدورديسيلا تاردا المعزولة من اسماك البلطي بتفاعل البلمرة المتسلسل تأثير زيت الثوم و المضادات الحيوية المختلفة على الميكروب المصنف خارجيا
Joint Authors
Ubayd, Sawsan Khamis Mahmud
Salim, Hibah S.
Source
Assiut Veterinary Medical Journal
Issue
Vol. 58, Issue 135 (13 Oct. 2012), pp.50-59, 10 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2012-10-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract AR
تم تجميع 130 عينة من سمك البلطى النيلي المستزرع السليم ظاهرياً و أجرى الفحص البكتيريولوجي للعينات لعزل ميكروب الأدورديسيلا تاردا و تصنيفه باختبار تفاعل البلمرة المتسلسل وقد كانت نسبة الأدورديسيلا تاردا التي تم عزلها من الأعضاء الداخلية للسمك (الكبد – الطحال – الأمعاء - الكلى – العضلات) 3.9 %.
و كذا تم إجراء اختبار الضراوة و الهيستوباثولوجي للميكروب المعزول و أيضاً اختبار الحساسية باستخدام المضادات الحيوية المختلفة و زيت الثوم كلاً على حدى و أيضاً المضادات الحيوية و زيت الثوم معاً و قد أوضحت النتائج أن ميكروب الأدورديسيلا تاردا الذي تم عزله مقاوم لزيت الثوم و معظم المضادات الحيوية.
كما أن زيت الثوم قلل من تأثير المضادات الحيوية المستخدمة في هذه الدراسة هذا و قد تمت مناقشة النتائج تفصيلياً.
Abstract EN
Edwardsiella tarda is considered as a serious pathogen of fish and it is also important due to its zoonotic aspects as infected fish processed for human consumption is a source of gastroenteritis and meningitis.
One hundred and thirty samples of cultured apparently healthy Nile tilapia were collected from private farms in Alexandria governorate and examined bacteriologically to isolate Edwardsiella tarda.
E.
tarda could be isolated from the kidney, liver, spleen, muscles and intestinal contents of examined fish with in a percentage of 3.
9 %.
The five isolates of E.
tarda were identified morphologically and biochemically then subjected to confirmation by conventional Polymerase Chain Reaction (PCR) with the amplification product of 415 bp using gyrB gene as a taxonomic marker.
Pathogenicity test of the isolate and histopathological examination of the experimentally infected fish were carried out.
In vitro, sensitivity test of the isolate against antimicrobials and garlic oil showed that it was resistant to garlic oil, Neomycin, Nitrofurantoin, Streptomycin, Tetracycline, Amoxicillin, Ampicillin/ sulbactam and Colistin; and sensitive to Chlormphenicol, Enerofoxacin, Flumequine Gentamycin ; while antimicrobials combined with garlic oil revealed that garlic oil decreased the potencies of antimicrobials.
proper aquarium hygiene, appropriate regulations of physical conditions in the aquarium are recommended and fish must be cooked sufficiently before eating to protect human health from such hazards.
American Psychological Association (APA)
Ubayd, Sawsan Khamis Mahmud& Salim, Hibah S.. 2012. Identification of edwardsiella tarda isolated from tilapia nilotica (oreochromis niloticus) by polymerase chain reaction (PCR). the effect of garlic oil and different antimicrobials on the identified organism in vitro. Assiut Veterinary Medical Journal،Vol. 58, no. 135, pp.50-59.
https://search.emarefa.net/detail/BIM-590001
Modern Language Association (MLA)
Ubayd, Sawsan Khamis Mahmud& Salim, Hibah S.. Identification of edwardsiella tarda isolated from tilapia nilotica (oreochromis niloticus) by polymerase chain reaction (PCR). the effect of garlic oil and different antimicrobials on the identified organism in vitro. Assiut Veterinary Medical Journal Vol. 58, no. 135 (Oct. 2012), pp.50-59.
https://search.emarefa.net/detail/BIM-590001
American Medical Association (AMA)
Ubayd, Sawsan Khamis Mahmud& Salim, Hibah S.. Identification of edwardsiella tarda isolated from tilapia nilotica (oreochromis niloticus) by polymerase chain reaction (PCR). the effect of garlic oil and different antimicrobials on the identified organism in vitro. Assiut Veterinary Medical Journal. 2012. Vol. 58, no. 135, pp.50-59.
https://search.emarefa.net/detail/BIM-590001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 57-58
Record ID
BIM-590001