Immunopathological study of the effect of bile salts on secondary hydatidosis development in white mice

Abstract EN

This study was carried out to investigate theimmunopathological effects of bile salts on the immune response against hydatid cyst infection in mice.The treated groups of mice dividedinto two groups,the first group immunized with 0.3ml of2mg/mlof bile salts and the second group immunized with 0.2ml of1mg / mlof bile salts in 0 time after two weeks the same doses as repeated against secondary hydatidatid cysts, then delayed type hypersensitivity test was done on these groups and compared with the third group(that had been inoculated with 0.2 ml of sterile phosphate buffer saline as a control group)at day 27 post immunization using soluble bile salts.At day 30 half number of animals from each group were scarified to perform humoral immunity tests (ELISA).Then challenge with 2000 protoscolices were done for the remaining half ofall 3groupsand left them for three months then kill them for histopathologicalresults post challenge showed marked growth of cysts in the livers of the control infected group, with some degenerative and necrotic lesions accompanied byamyloidosis in spleen.

While the immunized groups with bile saltsrevealed presence of focal mononuclear cellsinliver,kidney and lung tissue with lymphoid hyperplasiain spleen.Finally,this study showed that the bile saltswere highly immunogenicand this may related to the fact that one ofthecomponentsh of bile salts hasDeoxycholic acid init´s structure has the lysis effect on the protoscolsis against hydatid cyst infection in mice