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Expression and purification of the recombinant cytochrome P450 Cyp141 protein of mycobacterium tuberculosis as a diagnostic tool and vaccine production
Joint Authors
Haydari, Rida
Rabiee Furadnih, Muhammad
Darban Sarokhalil, Davud
Abdian, Narjis
Ihsan, A.
Alvandi, Amirhooshang
Sulaymani, Nada
Gholipour, Abu al-Fadl
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 17, Issue 6 (30 Jun. 2015), pp.1-6, 6 p.
Publisher
Publication Date
2015-06-30
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Abstract EN
Background : Tuberculosis (TB) is regarded as a health problem worldwide, particularly in developing countries.
Mycobacterium tuberculosis (M.
tuberculosis) is the cause of this disease.
Approximately two billion people worldwide are infected by M.
tuberculosis and annually about two million individuals die in consequence.
Forty million people are estimated to die because of M.
tuberculosis over the next 25 years if the measures for controlling this infection are not extensively developed.
In the vaccination field, Bacillus Calmette–Guérin (BCG) is still the most effective vaccine but it shows no efficacy in adult pulmonary patients.
One of the other problems regarding TB is its appropriate diagnosis.
Objectives : In this experimental study, the recombinant cytochrome P450 CYP141 protein of M.
tuberculosis was expressed and purified to be used as a vaccine candidate and diagnostic purpose in subsequent investigations.
Materials and Methods : The optimization of the cytochrome P450 CYP141 protein expression was evaluated in different conditions.
Then, this protein was purified with a resin column of nickel–nitrilotriacetic acid and investigated via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Western Blotting.
Results : The highest expression of the cytochrome P450 CYP141 protein was obtained by the addition of 1 mM of isopropyl β-D-1-thiogalactopyranoside (IPTG) to the bacterial culture grown to an optical density at 600 nm (OD600) of 0.6, 16 hours after induction.
This protein was subsequently purified with a purification of higher than 80%.
The results of Western Blotting indicated that the purified protein was specifically detected.
Conclusions: In this experimental study, for the first time in Iran the expression and purification of this recombinant protein was done successfully.
This recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations.
American Psychological Association (APA)
Haydari, Rida& Rabiee Furadnih, Muhammad& Darban Sarokhalil, Davud& Alvandi, Amirhooshang& Abdian, Narjis& Ihsan, A.…[et al.]. 2015. Expression and purification of the recombinant cytochrome P450 Cyp141 protein of mycobacterium tuberculosis as a diagnostic tool and vaccine production. Iranian Red Crescent Medical Journal،Vol. 17, no. 6, pp.1-6.
https://search.emarefa.net/detail/BIM-594193
Modern Language Association (MLA)
Haydari, Rida…[et al.]. Expression and purification of the recombinant cytochrome P450 Cyp141 protein of mycobacterium tuberculosis as a diagnostic tool and vaccine production. Iranian Red Crescent Medical Journal Vol. 17, no. 6 (Jun. 2015), pp.1-6.
https://search.emarefa.net/detail/BIM-594193
American Medical Association (AMA)
Haydari, Rida& Rabiee Furadnih, Muhammad& Darban Sarokhalil, Davud& Alvandi, Amirhooshang& Abdian, Narjis& Ihsan, A.…[et al.]. Expression and purification of the recombinant cytochrome P450 Cyp141 protein of mycobacterium tuberculosis as a diagnostic tool and vaccine production. Iranian Red Crescent Medical Journal. 2015. Vol. 17, no. 6, pp.1-6.
https://search.emarefa.net/detail/BIM-594193
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6
Record ID
BIM-594193