Surveillance of carbapenem non-susceptible gram negative strains and characterization of carbapenemases of classes A, B and D in a Lebanese Hospital
Joint Authors
Hammudi, Dalal
Mubarak, Carroll Ayyub
Kansu, Abir
Sarkis, Dolla Karam
Nordmann, Patrice
Source
Issue
Vol. 63, Issue 2 (30 Jun. 2015), pp.66-73, 8 p.
Publisher
Publication Date
2015-06-30
Country of Publication
Lebanon
No. of Pages
8
Main Subjects
Topics
Abstract EN
The production of carbapenem-hydrolyzing enzymes has been recognized as one of the most currently relevant resistance mechanisms in gram negative bacterial isolates, and is being detected in various countries.
In Lebanon, carbapenem resistance was studied among gram negative pathogens collected from a university hospital from January to June of years 2011 and 2012.
All isolates were subjected to phenotypic tests including antibiotic susceptibility, cloxacillin effect, modified Hodge test, and Etest® for metallo-β-lactamase detection.
They were also subjected to genotyping by PCR sequencing to characterize β-lactamases.
Between January and June 2011, 48 carbapenem non-susceptible strains were collected.
Of these, one Klebsiella pneumoniae harbored OXA-48 and insertion sequence IS1999; four Acinetobacter baumannii harbored simultaneously OXA-23 and GES-11, and three Pseudomonas harbored VIM-2 carbapenemase.
Between January and June 2012, 100 carbapenem nonsusceptible strains were collected.
Of these, one K.
pneumoniae harbored simultaneously OXA-48, IS1999, and an acquired AmpC of the ACC group; four Serratia marcescens harbored OXA-48, while among eight A.
baumannii, one strain co-harbored OXA-23 and GES-11, six harbored OXA-23 and one OXA-24.
Fifteen P.
aeruginosa and two Pseudomonas species harbored VIM-2 ; two P.
aeruginosa strains produced IMP-1 and two others IMP-2.
This epidemiological survey demonstrates the presence of carbapenemases of Ambler classes A, B, and D in a Lebanese hospital and indicates increase in the number and variety of such enzymes.
American Psychological Association (APA)
Hammudi, Dalal& Mubarak, Carroll Ayyub& Kansu, Abir& Nordmann, Patrice& Sarkis, Dolla Karam. 2015. Surveillance of carbapenem non-susceptible gram negative strains and characterization of carbapenemases of classes A, B and D in a Lebanese Hospital. Journal Médical Libanais،Vol. 63, no. 2, pp.66-73.
https://search.emarefa.net/detail/BIM-594663
Modern Language Association (MLA)
Hammudi, Dalal…[et al.]. Surveillance of carbapenem non-susceptible gram negative strains and characterization of carbapenemases of classes A, B and D in a Lebanese Hospital. Journal Médical Libanais Vol. 63, no. 2 (Apr. / Jun. 2015), pp.66-73.
https://search.emarefa.net/detail/BIM-594663
American Medical Association (AMA)
Hammudi, Dalal& Mubarak, Carroll Ayyub& Kansu, Abir& Nordmann, Patrice& Sarkis, Dolla Karam. Surveillance of carbapenem non-susceptible gram negative strains and characterization of carbapenemases of classes A, B and D in a Lebanese Hospital. Journal Médical Libanais. 2015. Vol. 63, no. 2, pp.66-73.
https://search.emarefa.net/detail/BIM-594663
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 72-73
Record ID
BIM-594663