Comparison of HLA class I and II molecular and serological typing within clinical laboratory
Joint Authors
Ayid, Khalid
Jendoubi, S. Ayed
Makhluf, M.
Sfar, I
Bin Abd Allah, Tayyib
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 14, Issue 1 (28 Feb. 2003), pp.39-42, 4 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2003-02-28
Country of Publication
Saudi Arabia
No. of Pages
4
Main Subjects
Topics
Abstract EN
In order to develop an adequate tissue typing strategy, we performed human leukocyte antigen (HLA)-A, B and DR generic typing on 235 (120 HLA-A, B and 115 HLA-DR) routine clinical samples by polymerase -chain reaction (PCR)-SSP in parallel with conventional serological typing.
At the A locus, there were two (1.7%) discrepancies between molecular and serological typing besides 25 (20.8%) serological blanks, which was defined by molecular typing.
At the B locus, there were two (1.7%) discrepancies and 30 serological blanks, which were defined by molecular typing.
At the DR locus there were two (1.8%) discrepancies and 44 serological blanks, which were defined by molecular typing.
We conclude that molecular typing is of substantial benefit in the resolution of poorly defined serological antigens.
In view of the low percentage of discrepancy between the serological and molecular typing besides the high cost of molecular typing, our policy is to perform HLA typing first by the serological method and to use PCR-SSP as an adjuvant tool.
American Psychological Association (APA)
Ayid, Khalid& Jendoubi, S. Ayed& Makhluf, M.& Sfar, I& Bin Abd Allah, Tayyib. 2003. Comparison of HLA class I and II molecular and serological typing within clinical laboratory. Saudi Journal of Kidney Diseases and Transplantation،Vol. 14, no. 1, pp.39-42.
https://search.emarefa.net/detail/BIM-59580
Modern Language Association (MLA)
Ayid, Khalid…[et al.]. Comparison of HLA class I and II molecular and serological typing within clinical laboratory. Saudi Journal of Kidney Diseases and Transplantation Vol. 14, no. 1 (Dec. 2003), pp.39-42.
https://search.emarefa.net/detail/BIM-59580
American Medical Association (AMA)
Ayid, Khalid& Jendoubi, S. Ayed& Makhluf, M.& Sfar, I& Bin Abd Allah, Tayyib. Comparison of HLA class I and II molecular and serological typing within clinical laboratory. Saudi Journal of Kidney Diseases and Transplantation. 2003. Vol. 14, no. 1, pp.39-42.
https://search.emarefa.net/detail/BIM-59580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 42
Record ID
BIM-59580