Using multiplex PCR assay for detection of genital mycoplasmasand ureaplasmaspp in an infertility males

Abstract EN

Objective : This study aim to diagnosis the types of bacteriaUreaplasmasppand Mycoplasma hominis by screening for multiple banding antigen (MBA) gene and 16S rRNA respectively in seminal fluid by using the multiplex-PCR technique.

Methodology : The study included , a total (106) semen fluid samples, were collected from 86 infertile men which have been diagnosed by specialized clinicians specialization in infertility and 20 other semen fluid samples from healthy men used as control through six months (November 2013 until April 2014).

All these Date was analyazed by using spss(T-test) program All these samples were obtained from subjects who attended to center infertility in medical city at Al-Sadar in Najaf province.

Results : The result of the multiplex-PCR revealed positive results in 13(15.1 %) of the 86 seminal fluid samples from infertile patients which represented M.hominis 5(5.8%), U.urealyticum in 5(5.8 %) and U.parvum3(3.5 %).

Conclusion : in light of the results obtained this study we concludes that the efficiency of multiplex-PCR large and rapid in diagnosis of Ureaplasmaspp and Mycoplasma hominis in seminal fluid.

Recommendation : the study of detection of other microorganism (viruses, fungi, Chlamydia and Neisseria gonorrhea) in men infertility.