Molecular characteristics of multidrug resistant Acinetobacter baumannii isolated from Baghdad hospitals
Other Title(s)
الخصائص الجزيئية لبكتريا Acinetobacter baumannii متعددة المقاومة للمضادات الحيوية و المعزولة من مستشفيات بغداد
Joint Authors
al-Masudi, Khatam Khalis
al-Saffar, Janan Muhammad Jawad
Qandala, Nuha Juzif
Source
Issue
Vol. 56, Issue 2B (30 Jun. 2015), pp.1394-1399, 6 p.
Publisher
University of Baghdad College of Science
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract AR
شملت الدراسة 100 عينة سريرية و من مصادر مختلفة (الإدرار، القشع، مسحات الجروح، مسحات الحروق و مسحات الإذن( من مرضى مستشفيات مختلفة في بغداد، خلال الفترة الممتدة من كانون الأول 2013 حتى مارس .2014 شخصت 15 عزلة (15 %) تعود إلى Acinetobacter baumannii و كانت أعلى نسبة تشخيص تعود إلى مسحات الجروح (40 %) و تغايرت نسبة العزل في مصادر العينات الأخرى .اختبرت حساسية عزلات بكتريا Acinetobacter baumannii باتجاه 16 مضاد حيوي، أظهرت نتائج الاختبار أن جميع العزلات كانت متعددة المقاومة للمضادات الحيوية.
استخدم تقنيه Polymerase Chain Reaction Technique (PCR) للتحري عن امتلاك العزلات قيد الدراسة على الجينات التي تشفر إلىg (Oxacillinase) فوجد 13 عزلة تشفر للتسلسلISAba1 ، بينما 12 عزلة كانت تحتوي على bla oxa 51 gene أما bla oxa58 gene and bla oxa 143 gene أظهرت نتيجة سالبه لكل العزلات.
Abstract EN
A total of 100 clinical sample from (urine, sputum and swabs of wound , burn and ear) were collected from patients in different hospitals of Baghdad during the period from December 2013 to May 2014.
15 isolates (15 %) identified belong to Acinetobacter baumannii, swabs of wounds were represented in high percentage of A.baumannii isolates (40 %) while percentage of other samples were variable.
Susceptibility of 15 A.baumannii isolates were tested toward 16 different Antimicrobial agents, the results showed all isolates were multi drug resistant.
In addition, Polymerase Chain Reaction Technique (PCR) was performed to detection the resistance genes encoding the Oxacillinases enzymes.
The PCR analysis showed that the presence of insertion sequence (ISAba1) in 13 isolates whereas blaoxa51like gene was represented in 12 isolates.
Furthermore, the results of detection other genes were not appear any amplification for all A.baumannii isolates with genes encoded for bla oxa 58 and bla oxa 143 enzyme.
American Psychological Association (APA)
al-Masudi, Khatam Khalis& al-Saffar, Janan Muhammad Jawad& Qandala, Nuha Juzif. 2015. Molecular characteristics of multidrug resistant Acinetobacter baumannii isolated from Baghdad hospitals. Iraqi Journal of Science،Vol. 56, no. 2B, pp.1394-1399.
https://search.emarefa.net/detail/BIM-597284
Modern Language Association (MLA)
al-Saffar, Janan Muhammad Jawad…[et al.]. Molecular characteristics of multidrug resistant Acinetobacter baumannii isolated from Baghdad hospitals. Iraqi Journal of Science Vol. 56, no. 2B (2015), pp.1394-1399.
https://search.emarefa.net/detail/BIM-597284
American Medical Association (AMA)
al-Masudi, Khatam Khalis& al-Saffar, Janan Muhammad Jawad& Qandala, Nuha Juzif. Molecular characteristics of multidrug resistant Acinetobacter baumannii isolated from Baghdad hospitals. Iraqi Journal of Science. 2015. Vol. 56, no. 2B, pp.1394-1399.
https://search.emarefa.net/detail/BIM-597284
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-597284
