The usage of in-pouch TV culture system as a method for trichomonas vaginalis detection in Baghdad-al-Karkh

Dissertant

Saba, Ayat Muhammad

Thesis advisor

al-Qadhi, Ban Nuri

Comitee Members

al-Shinawy, Fawziyah A.
Abd al-Rahman, Ban A.
Saghir, Khawlah H.

University

University of Baghdad

Faculty

College of Science

Department

Department of Biology

University Country

Iraq

Degree

Master

Degree Date

2012

English Abstract

Trichomonas vaginalis Donne,1836, accounts for almost half of all curable sexually transmitted infections and has also been associated with adverse outcomes of pregnancy and increased risk of HIV.

Therefore , accurate diagnosis important and cost-effective .

This study was carried out during the period from October ,2010 to April, 2011 to compare the use of a newer diagnostic culture system , In-Pouch (Biomed Diagnostics, USA)to different classical detection methods used in Iraq , and to evaluated the recent prevalence of trichomoniasis accordance with studying different factors that affect on vaginal trichomoniasis in females complaining of vaginal discharge .

Vaginal swabs and urine samples were collected from 200 women complaining vaginal discharge attending the Obstetrics and Gynecology out -patient department of AL-Yarmok Hospital, AL-Noor training Health Center and Privet Clinic in AL Shoulla city in Baghdad –AL-Khark .

Their age was ranging from 16-55 years.

The vaginal swabs examined by wet mount and cultivated in different culture media (In-Pouch TV system, OLM culture and Trichomonas Modified CPLM) to detect the presence of T.

vaginalis .

The pH strips and whiff test were used to detect the vaginal acidity and odor respectively.

Urine samples were ,examined as well by General Urine Examination to detect the presence of T.

vaginalis.

Of the 200 women,42 were positive by In-Pouch TV culture giving the prevelance rate of (21%) with sensitivity and specifiaty 100% .

In-Pouch TV system was found to be a good tool to detect T.

vaginalis with more sensitivity and accurate rate than other detection methods used .

Women aged 16-25 years had significantly higher prevalence of trichomoniasis (29.5%) than other age groups.

Summary II Married women had the highest percentage of trichomoniasis (25.8%), comparing with widowed women (6.1%).

Statistical significant difference was detected between these groups.

Multipara women revealed the highest rate of trichomoniasis (21.8%) followed by primipara group (17.9%) and finally infertile group (15.0%).

No statistical significant difference was detected between infection and parity.

Educated women had significantly higher rate of T.vagnalis infection (32.6%) than Illiterate women (11.1%).

Women with vaginal pH 6 , 5.5 and 5 had significantly higher rate of infection (63.1% , 32.1% and 44.4% respectively).

Women who used (pills and device) contraception had the highest rate of infection(54.7%) than those not using any contraception(45.3%).

No statistical significant differences were detected between these two groups.

Women with no clinical signs had the higher percentage of infection 34(80.9%) than women with vaginal erthrema and vulval erthema 5(11.9%), 3(7.1%) respectively.

No statistical significant differences were detected between these groups.

Women with yellowish discharge color showed higher significantly percentage of infection 24(57.1%) than other with white color and colorless discharge 13(30.9%), 5(11.9%) respectively.However women complaining of both discharge and itching had significantly higher percentage 14 (33.3%) than other clinical symptoms studies (discharge with itching and dysuria , discharge only , discharge and dysuria).

III

Main Subjects

Zoology

No. of Pages

104

Table of Contents

• APA
• MLA
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Language

English

Data Type

Arab Theses

Record ID

BIM-597567