The effect of toxoplasmosis on the level of some male sex hormones in samples from national blood transfusion center-Baghdad

Other Title(s)

تأثير الإصابة بداء المقوسات الكوندية على مستوى بعض الهرمونات الجنسية الذكرية في عينات من المركز الوطني لنقل الدم-بغداد

Dissertant

al-Saadii, Suhad Hasan Mahmud

Thesis advisor

al-Rawi, Khawlah Hawari Zughayr
al-Qadi, Ban Nuri

Comitee Members

Muhsin, Salwa Sabr
al-Alujy, Sabah Nasir
al-Shanawi, Fawziyah Ahmad

University

University of Baghdad

Faculty

College of Science

Department

Department of Biology

University Country

Iraq

Degree

Master

Degree Date

2013

English Abstract

Toxoplasma gondii is a unique intracellular parasite, which infect a large proportion of the worldP,Ps population, but clinically un commonly causes significant disease.

The present study was performed to estimate the prevalance of toxoplasomsis in Iraqi men and to examine the effect of toxoplasmosis infection on testosterone (total and free) and follicle stimulating hormone (FSH) changes in their sera.

Venous blood samples were collected from apparently healthy men they have ages between (18-57) years attended the National Blood Transfusion Center, during the period from October 2011 to Jaunary 2012.

Latex agglutination test (LAT) and Enzyme linked Immunosorbant Assay (ELISA), were used to evaluate the presence of anti-Toxoplasma IgM and IgG antibodies.

The levels of testosterone (free and total) and FSH hormones were also tested in men sera by ELISA test.

The results showed significant differences between seropositive of toxoplasmosis infections between LAT, 136/400 (34%) and ELISA - IgG, 121/400 (30.25%).

Only 10 male blood donors scored IgM presence by ELISA gaving an incidental rate of acute toxoplasmosis of 2.5%.

The results revealed a significant difference between age groups, both age groups of (18-25) and (26-33) years were showed the highest percentages of infections while, the age group of (50-57) years showed the lowest.

The blood group phenotypes of the infected male blood donors highlight the role in toxoplasmosis infection and the AB blood group characterized by the highest significant percentage of infection.

Chronic and actue toxoplasmosis infection in married males included in this study were significantly higher, 101(83.47%) and 7(70%) respect- ively than unmarried males 20(16.52%) and 3(30%) respectively.

.

However married males showed significant difference between fertile and infertile infected males, they were 31(30.69%), 6 (85.71%) and 70 (69.30%), 1(14.28%) by ELISA IgG and IgM respectively.

Private worker males reveraled highly significant percentages of chronic and acute toxoplasmosis 91(75.20%), 2(20%) respectively compared with official worker, 30 (24.79%), 8 (80%) respectively.

Chronic seropositive toxoplasmosis were higher in males inhabited rural regions, 111(91.73%), than in males inhabited urban regions,10 (8.26%).

Both acute and chronic toxoplasmosis in males recorded higher signifi- cantly mean concetrations in both total testosterone hormone TTH, (12.188 ±0.73 ng/ml), (7.837± 0.52ng/ml) and free testosterone hormone FTH, (44.121±1.76pg/ml), (27.984±0.94pg/ml), in comparison to control group (6.455±0.48ng/ml) and (18.375±0.78 pg/ml) respectively.

The mean concentration of FSH revealed non-significant differences in both disease activities (6.41IU/ml ±0.47) and (6.515±0.51 IU/ml) respectively, in comparison to control group (6.780±0.61 IU/ml).

The present data showed that the age group of (26-33) years scored higher significant mean concentrations (16.46 ng/ml) and (62.16 pg/ml) for TTH and FTH respectively in actue infected males, as well as they were showed the highest rates (12.62 ng/ml) and (45pg/ml) in chronic infected males respectively.

However, males infected with acute and chronic toxoplasmosis at the age group of (18-25) years were character- ized by few hair abundance in their faces.

This study revealed that married males infected with acute and chronic toxoplasmosis showed significantly the lowest mean concentrations (6.98 ng/ml) and (2.37 ng/ml) of TTH respectively, while they were recored the highest mean concentrations (62.74 pg/ml) and (52.46 pg/ml) of FTH when they compared with un-married infected males.

The married infected males who have children scored the highest mean concentrations in both TTH and FTH, they were (11.72 ng/ml), (64.42 pg/ml) and (2.5 ng/ml) ,(60.52 pg/ml) in acute and chronic infected males respectively.

On the other hand FSH showed non-significant differences between age groups and in the apperance of hair abundance in male faces in both acute and chronic disease.

Finally, FSH recorded non-significant differences between married and un-marriad males in their mean concentrations (6.49lU/ml), (6.72 lU/ml) and (6.33 lU/ml), (6.31 lU/ml) in acute and chronic infection respectively.

Males who have no childern showed significantly the highest mean concentrations of FSH, (7.20 lU/ml) and (7.26 lU/ml) for both disease activites (acute and chronic) respectively in comparison to male have children whom showed mean concentration of FSH, (5.78 lU/ml) and (6.18 lU/ml) for both disease activites respectively.

Main Subjects

Biology
Medicine

Topics

• Hormones
• Sex hormones
• Iraq
• Steroid hormones

No. of Pages

149

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-598171