Bacteriological and immunological study of streptococcus pneumoniae isolated from patients infected with respiratory tract infection in al-Najaf Governorate

Other Title(s)

دراسة بكتريولوجية و مناعية لبكتريا Streptococcus pneumoniae المعزولة من المرضى المصابين بالتهاب القناة التنفسية في محافظة النجف

Dissertant

al-Janabi, Ahmad Abd al-Jabbar Julub

Thesis advisor

al-Sadi, Abd al-Majid A.
Shnawa, Ibrahim Muhammad Salih

Comitee Members

al-Sadi, Muhammad A. K.
al-Musawi, Jafar K. N.
Muhal, Mahdi Husan
al-Ukayli, Khayri Abd Allah Dawud
al-Khatib, Ghazi Musa

University

University of Baghdad

Faculty

College of Science

Department

Department of Biology

University Country

Iraq

Degree

Ph.D.

Degree Date

2010

English Abstract

total number of 410 out patients attending the chest unit of AL-Sadder Teaching Hospital in AL-Najaf Governorate who were suspected of having respiratory tract infection from first February 2008 to the end January 2009 were included in this study with age range between 18-60 years old (males and females).

The sputum with Gram stain revealed the presence of lymphocytes were 190 specimens from a total of 410 specimens (46.341%) were positive, while the monocyte cells were present in 99 specimens (24.146%),the 3rd group of specimens is that with dominant neutrophils with 90 positive specimens (21.951%), the number of specimens positive for acid fast bacilli was 31 specimens (7.560%).

From the 90 specimens showing neutrophil cell domination, and the positive results for culture , microscopic and biochemical's characteristics for Streptococcus pneumoniae was 22 specimens only (24.444%), while 68 specimens (75.555%) were negative results for colonies by cultivation , microscopic and biochemical characterization for S.pneumoniae .

The results demonstrate that the most serotype distribution in AL-Najaf Governorate was serotype 6 while the serotype 1 was the most virulent isolate in Mice and Rabbits, thus it was chosen in this study because it is the most virulent one , and isolate 1 from serotype 6 because it is the most dominant serotype.

Three immunization protocols were used in this study to enhance specific immunoglobulin titer in rabbits.

The results demonstrates that the protocol 2 (rabbits intramuscular 1mg capsule mix with 1ml lanolin for 15 days) was the best method for enhancing systemic specific immunoglobulin of the rabbits, while protocol number 3 (Intranasal 1×108 cfu/ml of heat killed III S.pneumoniae for 30 days) was the best method for enhancing mucosal specific immunoglobulin of the rabbits, and the results indicate that capsular polysaccharides serotype 1 gave the highest response than serotype 6, and the statistical analysis proved that specific immunoglobulin titer in systemic was highest than mucosal washing in both for serotype 6 and 1 was significant at p value = 0.0007,on the other hand there was significant increase in specific immunoglobulin titer in serotype 1 systemic than serotype 6 systemic was significant at p value =0.0339, also the results indicates that the immunized rabbits with intramuscular 1mg capsule serotype 1 mix with 1ml lanolin for 15 days provided 80% protection and with serotype 6 provided 60% protection in laboratory rabbits post 36 days of immunization and infection with live S.pneumoniae (immune protection), and serotype 1 provided specific immunoglobulin titer in systemic higher than serotype 6 systemic , p= 0.0425 and the specific immunoglobulin titer in systemic was highest than mucosal washing for both serotype 6 and 1 and was significant at p value = 0.0200 and 0.0032 respectively.

The levels of cytokines were detected by three protocols, the results indicate that the protocol 3 gave the highest level in systemic and mucosal preparation of all cytokine levels ,then protocol 2 and 1 respectively, and the results indicate through protocol 2 for serotype 1 systemic there was no significant difference between TNFβ / TNFα , TNFβ / IL-6 , TNFα / IL-6 and IL-6 / IL-10, while it was significant between TNFβ / IL-10 (p=0.0342) , TNFα / IL-10 (p= 0.0018 ) and the results indicate for serotype 1 mucosal there was no significant difference between TNFβ / TNFα and IL-6/ IL-10, while there was significant difference between TNFβ / IL-6(p= 0.0004) , TNFβ / IL-10(p=<0.0001) , TNFα / IL-6(p=0.0060) and TNFα / IL- 10(p=0.0037),on the other hand the results indicate for serotype 6 systemic IV there was no significant difference between TNFβ / TNFα , TNFβ / IL-6 , TNFβ / IL-10 and IL-6 / IL-10,while there was significant difference between TNFα/IL-6(p=0.0019) and TNFα /IL-10(p=0.0305),also the results indicate that for serotype 6 mucosal preparation there was no significant difference between TNFβ /IL-6 , TNFβ /IL-10 and IL-6/ IL-10,however there was significant difference between TNFα / TNFβ (p=0.0027 ), TNFα / IL- 6(p=0.0007 ) and TNFα / IL-10(p=0.0001 ).

The results demonstrate that all cytokine levels post 36 day of immunization and challenge (immune protections) increased significantly than in controls and than lanolin, and the most significant increase was in mucosal than systemic it was the highest level of all cytokines.

On the other hand the results indicate that there was no significant increase for all cytokines except TNFβ and IL-10 (p=0.0447) in serotype 1 systemic, and there was a significant increase in TNFα and IL-6 , IL-10 (p= <0.0001 , 0.0004) respectively, also in IL-6 and IL-10(p=0.0038) serotype 1 mucosal ,on the other hand the results indicate that there was no significant increase for all cytokines levels for serotype 6 systemic and was significant for TNFα and IL-10(p=0.0173), and for IL-6 and IL-10(p=0.016) for serotype 6 mucosal.

Main Subjects

Medicine

Topics

• Microbiology
• Iraq

No. of Pages

136

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-598397