Molecular epidemiology of vibrio cholerae in Iraq during outbreaks 2007 to 2009

Other Title(s)

الوبائية الجزيئية لضمات الكوليرا V.cholerae في العراق خلال فترات الأوبئة 2007-2009

Dissertant

al-Naddawi, Tahrir Hadi Salih

Thesis advisor

Hamad, Zuhayr Numan
Jasim, Kifah Ahmad

University

University of Baghdad

Faculty

College of Science

Department

Department of Biology

University Country

Iraq

Degree

Ph.D.

Degree Date

2010

English Abstract

This study included bacteriologic, enzymatic and genetic sides for some virulence factors produced by 80 clinical V.

cholerae isolates of the biotype ELtor which be collected from the Central Public Health Laboratory in Baghdad, as well as, five isolates of environmental source collected from different water rivers in Baghdad and other provinces.

The Vibrios were diagnosed primarily by subjecting them to microscopic and biochemical test .API 20 E was confirmely used to diagnose all clinical and environmental isolates.

Then antisera were used to specify serotypes and subserotypes.

To specify if the strains were belonging to the biotypes ELtor or classical, some biochemical test were applied.

A modern diagnostic system, which included genetic test, it was done by using simplex PCR profile which revealed that the ompW gene.

This test was employed for the first time in our country.

The results revealed that forty four (44) (55%) of eighty (80) clinical isolates were Inaba serotype, twenty six (26) (33%) belonged to the serotypes Ogawa.

Out of remaining isolates, ten (10) (13%) isolates belonged to the serotype Non-O1.

All environmental V.

cholerae isolates were belonged to the serptype Non/O1, 3 isolates belonged to V.

parahaemolyticus, 4 isolates belonged to V.

valnificus, one isolate belonged to V.

fluvialis and V.

alginolyticus and finally 6 isolates belonged to non Vibrio genus (A.hydrophila).

The Vibrios isolates were subjected to antimicrobial sensitivity, it was performed for som antibiotics and for vibriostatic (O129), in this study, the results showed that all environmental isolates were sensitive to all antimicrobial agents, while all clinical isolates showed a high sensitivity (100%) to Ampicillin, Gentamycin, Cepalothin, Tetracyclin, Erthromycin and Ciprofloxacin, and showed a high resistance (97.5%) to Cotri-moxazole, Nalidixicacid and Chloramphenicol.

It was found that all V.cholerae (O1) IV isolates were resistance to the Vibriostatic O129 and all Non/ O1 V.cholerae isolates were sensitive to the Vibriostatic O129.

All clinical isolates produced necrotic effect by skin rabbit test and one isolate (E5) of environmental isolates gave the same action, during this test a highly relationship between protease enzyme and entrotoxin production.

All clinical and environmental were produced hemolysin, while they showed variety rates of producing protease and lipase enzymes.

Quantitative analysis for TCP toxin showed that all clinical isolates O1 and non/O1 showed a positive results (autoagglutination in vitro), while only one isolates (E5) had the same result.

PCR analysis gave indication that all clinical and environmental isolates gave a positive results for the existence of toxR gene.

While only clinical isolates (O1) were positive for possessing of ctxA, ctxB, tcpA genes.

From the epidemiological view, all isolates participated to all outbreaks of 2007, 2008 and 2009 belong to the same source as far as, a PCR profile is concerned.

Plasmid profile for Vibrio cholerae isolates in the current study was studied by using WizardÒ plus SV minipreps DNA purification kit.

The results showed that the mijaroty of Vibrio isolates were free of plasmids.

Main Subjects

Biology

Topics

• Epidemiology
• Cholera
• Iraq

No. of Pages

141

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-598485