Detection of toxoplasmosis by using latex agglutination test and enzyme linked immunosorbent assay in Mayssan Province Ewes

Abstract EN

Venous blood samples were collected 283 ewes observed in nine different regions of Maysan province during the period from October 2012 to March 2013 for detecting toxoplasmosis by using Enzyme linked immunosorbent assay (ELISA-IgG) and latex agglutination test (LAT).

Three groups of ewes pregnant, non-pregnant and aborted were selected for both tests, and the results showed that the rates of Toxoplasma seropositivity in aborted group is the highest, followed by pregnant group using the LAT test (73.33 % and 65.38 %) respectively, while the proportion of ewes aborted were the top (40 %) in the ELISA test.

The results of LAT and ELISA tests showed that Al–Musharah subdistrict is the highest in the seropositivity against Toxoplasma (78.26 % and 39.13 %) respectively.

As for the relationship between age and Toxoplasma seropositivity, the results showed that the highest seropositivity rate in the age groups more than a year and two years (65.30 % and 65.57 %) respectively by using LAT test, and high seropositivity rate (32.65) in the age group more than a year with ELISA test.

The statistical analysis showed no significant differences(p ≥ 0.05) between the LAT and ELISA tests with regard to Toxoplasma seropositivity.