Detection of cadmium in soil and parsley petroselinum crispum with studying their effect on some factors of oxidant-antioxidant system in male albino mice

Other Title(s)

الكشف الكشف عن الكادميوم في التربة و نبات المعدنوس Petroselinum crispum و دارسة تأثيرهما في بعض عوامل نظام الأكسدة و مضاداتها في ذكور الفئران البيض

Dissertant

Yusuf, Huda Abd al-Masih

Thesis advisor

al-Zubaydi, Fawzi Shanawah
Yusuf, Walid Hamid

Comitee Members

Abd al-Hadi, Faris Naji
Ali, Muhammad Nifi
Anun, Muhammad R.
Jawad, Abd al-Latif Muhammad

University

University of Baghdad

Faculty

College of Science

Department

Department of Biology

University Country

Iraq

Degree

Ph.D.

Degree Date

2013

English Abstract

This study was conducted to investigate the interaction effect between parsley Petroselinum crispum and cadmium on some factors of oxidant-antioxidant system in male albino mice as a laboratory animal model.

To achieve this study, the first step was to measure cadmium levels in local farmed parsley in some agricultural areas of Baghdad city and cadmium concentration in their soil in order to detect the pollution with cadmium and choose the experimental concentrations that simulate cadmium concentrations that already exist in parsley.

The survey study revealed that cadmium concentrations in soil samples were higher than parsley samples.

Cadmium concentrations in both soil and parsley from the north of Baghdad city agricultural areas (Al-Rashdiya, Tunis quarter and Al-Shamasiya quarter) were lower than those from the middle (Al-Attaifiya) and south (Al-Tuaithah) of the city.

The lowest cadmium concentration in soil and parsley was recorded in Tunis quarter 1.25 and 0.26 mg/kg, respectively; while the highest soil concentration of cadmium was in Al-Attaifiya 3.54 mg/kg and it recorded the highest cadmium concentration in parsley (0.39 mg/kg).

These results showed that cadmium concentrations in soil and plant samples were exceeded the references limits.

The detection of some active compounds in parsley showed that parsley contains flavonoids, saponins, alkaloids, tannins, terpenes and steroids.

The second part of the study was to examine the effect of cadmium in male mice and the interaction effect between cadmium and parsley; cadmium and vitamin C (vitamin C was used as a pure antioxidant).

One hundred and fifty healthy mature albino male mice at the average age of 8-10 weeks and weight of 25±5 g were randomly divided into 15 groups of ten animals for each group.

Five cadmium concentrations (0.0, 0.25, 0.5, 0.75 and 7.5) mg Cd/kg b.w., were selected to be administered to mice orally using gavages needle for 60 days at the dose of 0.1 ml.

The first five groups of mice were administered with cadmium alone.

The second five groups were administered 0.1 ml of vitamin C at the concentration of 500 mg/day with the five cadmium concentrations.

The last five groups of mice were administered 0.1 ml of parsley juice with the five cadmium concentrations.

The results showed a significant (P<0.05) increase in serum cholesterol, triglycerides and low density lipoproteins (LDL); and non-significant (P>0.05) changes in high density lipoproteins (HDL) after cadmium administration.

The administration with vitamin C or parsley significantly (P<0.05) decreased serum cholesterol and LDL with a non-significant (P>0.05) changes in triglycerides, while HDL showed significant (P<0.05) changes at cadmium concentrations of (0.5 and 0.75) mg Cd/kg b.w.

The present study showed a significant (P<0.05) decrease in serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase (GPT and GOT) activities according to vitamin C or parsley administration, while cadmium concentrations alone caused a significant (P<0.05) increase in serum GPT and GOT activities.

The results also revealed a significant (P<0.05) and concentration dependant increase in cadmium bioaccumulation in the liver and kidney, while the administration of vitamin C or parsley decreased cadmium concentrations in both liver and kidney significantly (P<0.05).

The results of the present study showed a significant (P<0.05) decrease in malondialdehyde (MDA) levels with a significant (P<0.05) increase in catalase activity in both liver and kidney after the administration of vitamin C or parsley to cadmium-treated mice.

Cadmium alone increased MDA levels significantly (P<0.05) and decreased catalase activity significantly (P<0.05) in both liver and kidney.

The histological sections showed different changes in liver and kidney structures.

Congestion, Kupffer cells hyperplasia, necrosis, sinusoidal dilation and cholesterol accumulation were observed in liver sections after cadmium intoxication, while kidney showed degeneration and damage in renal tubules and necrosis in epithelial cells.

Vitamin C and parsley showed some improvements in the structure of liver and kidney; retreated glycoprotein granules accumulation, normal appearance of liver structure; normal glomeruli and renal tubules were observed in kidney sections.

Li

Main Subjects

Biology
Zoology

Topics

• Physiology
• Oxidative Stress
• Mice
• Cadmium

No. of Pages

146

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-598774