Prevalence of Aero monas spp. in raw and drinking water, and detection of their ability to form biofilm
Other Title(s)
انتشار بكتريا .Aeromonas spp النيابة في المياه الخام و مياه الشرب و التحري عن قابليتها لتكوين الأغشية الحيوية
Dissertant
Thesis advisor
Comitee Members
Abd al-Jalil, Sana Burhan
al-Maadhidi, Jabbar F.
Kadhim, Shuruq R.
al-Musawi, Rasmia A.
Hassani, Hayfa Hadi
Muslih, Rashid Mahjub
University
University of Baghdad
Faculty
College of Science
Department
Department of Biology
University Country
Iraq
Degree
Ph.D.
Degree Date
2013
English Abstract
A total of 551 water samples (drinking and raw water)were collected during the period April 2011 till February 2012.In this study , Aeromonas spp were detected by biochemical tests and PCR(16s rRNA gene) also hemolysin genes (hemolysin and aerolysin) were detected.
This study investigated the effects of several factors on Aeromonas hydrophila isolates and on their ability to produce biofilm on four solid surfaces which used in pipe distribution systems .
The percentages of Aeromonas recovery from river water was 72.52%, and from wells water was 35% .Total percentage of positive aeromonas samples in treated water(Filtration &chlorine tank , supply water of WTPs, distribution system, reserviores(house tanks) and other samples was 8.8%.
The results of identification to species are : the most predominant species was A.
hydrophila 63 isolates(49.21%) , A.
trota 21 isolates(16.41%), A.
veroni 12 isolates(9.37%) A.
salmoncida 11 isolates(8.59%),and A.
schubertii, A.
sobria, A.
cavia four isolates for each(3.13%) , Aeromonas SPP nine isolates(7.03%).
Aeromonas hydrophila isolates revealed higher resistant for most antibiotics used in this study, also higher resistant to heavy metal salts.
Other part of this work is a study the ability of A.hydrophila to form biofilm.
The more two efficient isolates(drinking water isolate DW3,and raw water isolate RW1) which produce biofilm was chosen to study the effect of different values of temperature and pH on the growth of these bacteria and their ability to adhere to four solid surfaces( stainless steel (SS) ,galvanized iron(GI), polyvinyl chloride (PVC) and unplasticised polyvinyl chlorideUPVC )under different conditions .Results of current study showed that higher number of A.hydrophila that adhered was found on uPVC follows by the PVC while SS and GI was the least in ability of attraction to bacteria at 37°C.A.hydrophila (DW,RW)isolates has the ability to adherence on four solid surfaces at low temperature ٥°C greater than 37°C.
The MIC values varied according to the type of disinfectants in the range (1550-10000) μg/ml.
In this experiment, dettol appeared highest activity as disinfectants against both isolates of A.
hydrophila(DW,RW) recording a minimum value of MIC (1550) μg/ml, follows by bleach with MIC(7500) μg/ml, while the maximum value of MIC (10000) μg/ml was observed with iodine .The bleach at MIC concentration was the most efficient disinfectants than other disinfectants in its ability to remove the adherent bacteria on solid surfaces followed by dettol , then iodine solution.
Molecular analysis was included several points : -Detection of Aeromonas spp by 16s rRNA gene .
The sequencing results of the 16s rRNA gene(bionear, Seoul- Korea) have shown that high sequence homologies exist between the 16s rDNA gene and GenBank database.
Sequencing of PCR products confirmed the presence of some Aeromonas species such as A.
enteropelogenes for the first time in Iraq .
Moreover results showed that two isolates of Aeromonas hydrophila(local isolates) had percentage similarities 100% with Aeromonas hydrophila ATCC 7966 in GenBank database.
-A simplex PCR assay was performed to determine whether a597 bp hlyA gene and 416bp aerA gene fragments could be detected in sixty three Aeromonas isolates.
Four genotypes among A.
hydrophila isolates were found in this study ,the hlyA+ aerA+ genotype was the most common genotype.
-A survey was performed on the sixty three A.
hydrophila isolates by duplex PCR to detect hemolysin genes presence.
Four genotypes among A.
hydrophila isolates also were found.When the presence of both toxin genes was investigated the hlyA+ aerA+ genotype was the most common ,50.79 %(32 isolates ).
Main Subjects
Topics
No. of Pages
183
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Introduction.
Chapter One : Literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussion.
References.
American Psychological Association (APA)
Ulaywi, Sana Rahman. (2013). Prevalence of Aero monas spp. in raw and drinking water, and detection of their ability to form biofilm. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-598930
Modern Language Association (MLA)
Ulaywi, Sana Rahman. Prevalence of Aero monas spp. in raw and drinking water, and detection of their ability to form biofilm. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad. (2013).
https://search.emarefa.net/detail/BIM-598930
American Medical Association (AMA)
Ulaywi, Sana Rahman. (2013). Prevalence of Aero monas spp. in raw and drinking water, and detection of their ability to form biofilm. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-598930
Language
English
Data Type
Arab Theses
Record ID
BIM-598930
