Molecular Study on cystic Echinococcosis in some Iraqi patients
Other Title(s)
دراسة جزيئية على مرضى الأكياس المائية في بعض المرضى العراقيين
Dissertant
Baraak, Muhmmad Jubayr Muhaidi
Thesis advisor
al-Hassani, Hayfa Hadi
al-Qadi, Ban Nuri
Comitee Members
Saghir, Khawlah H.
Jasim, Hamid M.
al-Rabii, Salwa S.
al-Azzawi, Athmar Khudayr Abbas
al-Saqr, Ihsan Mahdi
University
University of Baghdad
Faculty
College of Science
Department
Department of Biology
University Country
Iraq
Degree
Ph.D.
Degree Date
2014
English Abstract
Human hydatid cysts were surgically removed from 30 patients who underwent surgery for hepatic, pulmonary, spleen, heart, and peritoneal cavity cystic echinococcosis (CE) at hospitals from various provinces of Iraq, between March 2012 and January 2013.
According to questionnaire sheet that used for demographic, epidemiologic, and clinical data for each patients, the result showed the incidence of CE was higher in females than in males and most infections 33.33% were at the age group (21-30) year.
The proportion of infection in rural areas were higher than urban, with most affected organ the liver 50%, cysts integrity characterized by high percentage (90%) of intact cyst.
Two components of hydatid cysts (germinal layer and protoscoleces) were used for DNA extraction by four different methods (mechanical grinder, boiling, freezing-thawing, and crushing by sonicator).
The amount of released DNA from protoscoleces was higher than germinal layer and mechanical grinding obtained best results for this process.
Purification of mtDNA was done by three procedures (manual extraction, promega kit, and genaid kit) and promega kit was selected for its advantages in comparison to the other two procedures.
The mitochondrial COX1 (cytochrome c oxidase subunit 1) and NADH dehydrogenase subunit 1 (ND1) genes were used as targets for polymerase chain reaction (PCR) amplification, all of 30 hydatid cysts yielded amplification products.
For CO1 primers produced 450 bp PCR product while for NADH1 primers produced 800 bp.
IV The PCR products were purified and partial sequences were generated.
The sequences obtained were found to align with corresponding region for CO1 and ND1 genes in the Gene Bank nucleotide database confirming the human cysts to contain genotype of sheep strain (G1) in Iraq.
The sequences were further aligned with the corresponding regions of CO1 and ND1 genes of known genotypes from other countries to compare the degree of matching especially with other countries and the results showed that the highest match (26.66%) of CO1 was with Mongolia.
While, for ND1 the highest match (70%) with Argentina.
Main Subjects
Topics
No. of Pages
169
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction and literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussion.
Conclusions and recommendations.
References.
American Psychological Association (APA)
Baraak, Muhmmad Jubayr Muhaidi. (2014). Molecular Study on cystic Echinococcosis in some Iraqi patients. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-599044
Modern Language Association (MLA)
Baraak, Muhmmad Jubayr Muhaidi. Molecular Study on cystic Echinococcosis in some Iraqi patients. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad. (2014).
https://search.emarefa.net/detail/BIM-599044
American Medical Association (AMA)
Baraak, Muhmmad Jubayr Muhaidi. (2014). Molecular Study on cystic Echinococcosis in some Iraqi patients. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-599044
Language
English
Data Type
Arab Theses
Record ID
BIM-599044
