Study of Rnase enzymes in saliva samples from women with breast tumors
Other Title(s)
دراسة إنزيمات RNase في نماذج لعاب النساء المصابات بأورام الثدي
Dissertant
al-Isa, Yasir Abd al-Husayn Jafar
Thesis advisor
University
University of Baghdad
Faculty
College of Science
Department
Chemistry Department
University Country
Iraq
Degree
Master
Degree Date
2007
English Abstract
RNase activities in saliva of 30 patients with benign tumors, 33 patients with malignant tumors and 25 normal subjects were measured.
The first part of this study was devoted to:- 1.
The measurement of RNase activity (alkaline and acid) in saliva of control, patients groups.
The results indicated presence of a highly significant elevation (p<0.001) of these enzyme’s activities in cancer groups in comparison with those of other groups.
2.
Upon conventional electrophoresis of the crude saliva samples of the above three studied groups.
Differences in the patterns of proteins and RNase activity were found.
The second part of this study dealt with follow the changes and the alterations in saliva alkaline RNase using gel filtration chromatography as a tool.
The results revealed the presence of many peaks with RNase activity in the saliva of all studied groups.
All these peaks exhibited an activity, when the enzymatic reactions were carried out in Davis buffer at pH 8 and 5, except one peak which was separated from the saliva of malignant patients, which showed RNase activity only when the enzymatic assay was carried out at an alkaline buffer (pH=8).
The Summary VII The third part of this study dealt with the study of some properties of the two peaks which have been isolated by gel filtration chromatography from the saliva samples, and showed maximum RNase activity.
Such study included stability, determination of molecular weight, and effect of substrate concentration, pH value and cations on the activity.
Main Subjects
No. of Pages
92
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction and literature survey.
Chapter Two : Experimental work.
Chapter Three : Results and discussion.
Conclusion.
References.
American Psychological Association (APA)
al-Isa, Yasir Abd al-Husayn Jafar. (2007). Study of Rnase enzymes in saliva samples from women with breast tumors. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-599381
Modern Language Association (MLA)
al-Isa, Yasir Abd al-Husayn Jafar. Study of Rnase enzymes in saliva samples from women with breast tumors. (Master's theses Theses and Dissertations Master). University of Baghdad. (2007).
https://search.emarefa.net/detail/BIM-599381
American Medical Association (AMA)
al-Isa, Yasir Abd al-Husayn Jafar. (2007). Study of Rnase enzymes in saliva samples from women with breast tumors. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-599381
Language
English
Data Type
Arab Theses
Record ID
BIM-599381
