Phosphatases and osteodystrophy in chronic renal failure as related to oxidative stress

Other Title(s)

إنزيمات الفوسفاتيز ونقص التكوين العظمي في مرضى الفشل الكلوي المزمن و علاقته بجهد الأكسدة

Dissertant

al-Imami, Sura Ahmad Abd al-Sattar

Thesis advisor

Hasan, Hadhamh Razzuqi

University

University of Baghdad

Faculty

College of Science

Department

Chemistry Department

University Country

Iraq

Degree

Ph.D.

Degree Date

2007

English Abstract

Forty patients with ESRD underwent hemodialysis for (3- 24)months, aged (14-65) years attending Al-Karama Hospital and specialized surgery Hospital were included in the present study, also thirty seven healthy individuals of matched age and sex were utilized as control throughout this study.

The first part of this study is devoted to the measurement of activities and specific activities of total acid phosphatase ACP, tartrateresistant acid phosphatase (TRACP), and prostatic acid phosphatase (ACP) in sera of control and patients with ESRD.

Different results in the mean values of these enzymes activities and specific activities (P > 0.05) were observed in sera of patients with ESRD in comparison with that of control group.

However upon analysis the results of enzymes activity in sera of patients with ESRD.

The following was found:- · Significant increase (P < 0.001) in TRACP activity in sera of 25% of patients with ESRD.

· Significant increase (P < 0.001) in activities of both TRACP and PAP in sera of 5% of patients with ESRD.

· Significant decrease (P < 0.001) in activities of both TRACP and PAP in sera of 35% of patients with ESRD.

In the second part of this study, some biochemical parameters including alkaline phosphatase activity (ALP), testosterone, calcium, and phosphorus concentrations were measured in sera of control and patients with ESRD, to evaluate the presence of bone disease as a complication in the studied patients.

The results indicated the following:- · Significant increase (P < 0.01) in ALP activity and in phosphorus concentration(p<0.01) in sera of patients with ESRD in comparison with that of the control group.

XII · Significant decrease in calcium concentrations (P < 0.001) and testosterone level(p<0.01) in sera of patients with ESRD in comparison with that of the control group.

The overall results in this part of the present study enable us to classify the studied patients with ESRD into two groups as far as bone remodeling is concerned: group I with high turnover rate and group II with a change in their remodeling rate.

In the third part of this study some biochemical parameters including concentration of malondialdehyde (MDA), copper, iron, transferrin, ,uric acid, in addition to ceruloplasmin as ferroxidase activity were measured in sera of control and patients with ESRD to evaluate the oxidation- antioxidant status in the studied patients.

The results indicated the following:- · Significant increase (P < 0.001) in both MDA and uric acid level in sera of patients with ESRD in comparison with that of the control group.

· A non significant increase (P > 0.05) in serum of copper concentration of patients with ESRD in comparison with that of control group.

· Significant decrease (P < 0.001) in serum iron, TIBC, UIBC, and transferrin of patients with ESRD in comparison with that of the control group.

While no differences in the saturation percentage of transferring with iron was observed in sera of patients with ESRD in comparison with that of control group.

· Non significant decrease in both activity and specific activity of ceruloplasmin ferroxidase (P > 0.05) in sera of patients with ESRD in comparison with that of control group.

In the last part of this study, ion exchange chromatography was applied in order to purify TRACP isoforms (5a and 5b) from sera of XIII control and patients with ESRD to characterize both isoforms physicochemically and spectrophotometrically.

The results indicated the following: · TRACP 5a and 5b had different specific activities and their proportional amounts in sera of patients with ESRD were different from that of the control group.

· TRACP 5a and 5b had approximate molecular weight of (36.8-39.3 KDa) and a basic isoelectric point equal to 9.6 in control and patients with ESRD.

· The optimal for 5b is relatively higher than for 5a.

· The heparin (23 KU/L) had an inhibitory effect only on the activity of TRACP 5a .

· The iron content of isoform 5b was found to be one mole per one mole of enzyme.

· UV spectrum of TRACP 5a was found to be different from that of TRACP 5b in both control and patients with ESRD.

Main Subjects

Chemistry

Topics

• Chemistry
• Biology
• Oxidative Stress
• Kidneys
• Biochemistry
• Diseases
• Chronic diseases
• Kidney failure

No. of Pages

196

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-600264