Study of some biochemical changes in serum and saliva of patients with oral epithelial tumors

Other Title(s)

دراسة بعض المتغيرات الكيميائية الحياتية في مصل و لعاب المرضى المصابين بأورام الخلايا الطلائية للفم

Dissertant

Dawud, Rukzan Mahmud

Thesis advisor

Hasan, Hadhamh Razzuqi

University

University of Baghdad

Faculty

College of Science

Department

Chemistry Department

University Country

Iraq

Degree

Ph.D.

Degree Date

2008

English Abstract

The present comprehensive study was designed to investigate the changes in some biochemical parameters, in sera and saliva samples of patient with oral tumor (benign & malignant), and to establish the possibility of using saliva as a supplementary to serum for diagnosis and epidemiological testing of oral cancer.

Thirty three patients with oral tumors (benign & malignant) aged from 15 – 75 years attending the specialized surgery hospital in Baghdad medical city, were included in the present study.

Also thirty two healthy individuals of matched age and gender were utilized as control.

The main measurements included :- 1- Determination of sera and saliva contents of total proteins and then these proteins were analyzed using conventional electrophoresis technique.

Albumin and ceruloplasmin were also determined in sera and saliva samples; meanwhile determination of IgA, IgG and IgM was carried out on sera samples only.

2- Determination of the concentration of urea, uric acid, calcium, and phosphorus in sera and saliva samples of the studied groups.

3- Determination the activity and specific activity of Alkaline phosphatase, Lactate dehydrogenase, Amylase, Alkaline and Acid RNase, Ceruloplasmin oxidase, Total superoxide dismutase and Total peroxidase.

As well as the concentration of related trace elements such as Fe, Cu, Zn, and Mn was also measured.

4- Follow up the variation of these enzymes by using polyacrylamide gel electrophoresis technique.

II The observed results could be summarized as follows;- For malignant tumor group, the following results were obtained:- In Serum:- -- Significant increase in comparison with the control at level (P<0.001): in globulin concentration and alk.

RNase avtivity.

at Level (P<0.01): in total protein, ceruloplasmin concentration, IgA concentration and peroxidase activity.at level (P<0.05) in IgG concentration, uric acid, alk.

RNase specific activity ceruloplasmin oxidase activity, Zn and Mn concentration.

-- Non significant increase in comparison with the control at Level (P>0.05) in:- IgM concentration, urea, alkaline phosphatase activity & specific activity, amylase activity&specific activity, acid RNase activity&specific activity, superoxide dismutase activity ceruloplasmin oxidase specific activity, LDH specific activity zinc and copper concentration.

-- Significant decrease in comparison with the control at level (P<0.001) in: albumin level, and at level (P<0.05) in [Fe]/peroxidase activity and iron concentration.

Wherease a non significant decrease was shown in superoxide dismutase specific activity.

In Saliva:- -- Significant increase in comparison with the control at level (P<0.001) in peroxidase activity, at level (P<0.01) in total protein, urea, and copper concentration, at level (P<0.05) in ceruloplasmin concentration, peroxidase specific activity.

-- Non significant increase in comparison with the control at(P>0.05) was shown in:- globulin concentration, calcium concentration, alkaline phosphatase activity and specific activity, lactate dehydrogenase specific activity, alkaline RNase activity, acid RNase activity and III specific activity, superoxide dismutase activity, ceruloplasmin oxidase specific activity, and zinc concentration.

-- Significant decrease at level (P<0.001)in:- alpha amylase activity, manganese concentration, at level (P<0.05) in uric acid concentration, superoxide dismutase specific activity.

For benign tumor group, the following results were obtained:- In Serum: --Significant increase in comparison with the control at (P<0.001) in IgA concentration, alk RNase activity, peroxidase activity, at level (P<0.05) in: - globulin, alkaline phosphatase activity, amylase activity, lactate dehydrogenase activity&specific activity, alkaline RNase specific activity, and peroxidase activity.

--Non significant increase in comparison with the control at (P>0.05) was shown in: - total protein, ceruloplasmin concentration, IgM, IgG, urea and uric acid, calcium & phosphorus, acid RNase activity &specific activity, CP oxidase activity &specific activity, superoxide dismutase activity, peroxidase specific activity and copper, zinc and manganese concentration.

--Significant decrease at level (P<0.01) in iron concentration.

In Saliva: --Significant increase in comparison with the control at level (P<0.001) in phosphorus, iron and manganese concentration, at level (P<0.01) in globulin, at level (P<0.05) in albumin, ceruloplasmin concentration, LDH activity, SOD specific activity.

-- Non significant increase in comparison with the control at level (P>0.05) in total protein, urea, calcium, ceruloplasmin oxidase specific activity, peroxidase activity &specific activity, alkaline phosphatase specific activity, superoxide dismutase specific activity.

IV --Significant decrease at level (P<0.01) in uric acid, at (P<0.05) in amylase activity &specific activity.

pI values for peroxidase enzyme were: 5.76, 6.95, 8.1 in serum and 7.4, 8.4 in saliva.

pI value for superoxide dismutase enzyme was 3.8-6.2 in serum and saliva.

The results were revealed positive correlation between some parameters in sera and saliva samples of the three studied groups.

Main Subjects

Chemistry

Topics

• Chemistry
• Biology
• Mouth
• Biochemistry
• Neoplasms
• Diagnosis

No. of Pages

245

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-600362