The effect of different temperatures on the expression of hsp90 gene from the local isolate of Salmonella typhimuruim using RT-PCR
Other Title(s)
تأثير درجات حرارية مختلفة على شدة التعبير الجيني ل (hsp90) للعزلة المحلية لبكتريا Salmonella typhimurium باستخدام تقنية RT-PCR
Dissertant
Thesis advisor
Aziz, Ghazi Munim
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab
University
University of Baghdad
Faculty
College of Science
Department
Biotechnology Department
University Country
Iraq
Degree
Master
Degree Date
2014
English Abstract
Three bacterial isolates related to genus Salmonella obtained from the central health laboratory/ ministry of health /Baghdad.
Then these isolates were identified serologically by antisera test, from statins serum Institut (SSI) using the agglutination reaction (Antigen-antibodycomplex) to the following Salmonella typhimurium, salmonella typhi, salmonella enteridis.
The hsp90 gene was identified for the studied isolates by specific primers for gene amplification using PCR technique to include nucleotide sequence up and down stream gene.
Results of electrophoresis on agarose gel revealed that the approximate size of the gene is about 161bp.
The intensity of gene expression hsp90 for Salmonella typhimurium measured by CT when the bacterial isolate was exposed to different temperatures(30ºC, 40ºC,45ºC,50ºC,55ºC) using qRT-PCR compared to the normal growth temperature and to the internal positive control(Reference gene) 16sRNA.
Result showed a regular increasing of gene expression with temperature, untile a maximum intensity observed at 50ºC.
However the intensity at 55 was statistically insignificant.
Main Subjects
No. of Pages
98
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Introduction.
Chapter One : literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussions.
Conclusions and recommendations.
References.
American Psychological Association (APA)
Khudayr, Marwah Muhsin. (2014). The effect of different temperatures on the expression of hsp90 gene from the local isolate of Salmonella typhimuruim using RT-PCR. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601149
Modern Language Association (MLA)
Khudayr, Marwah Muhsin. The effect of different temperatures on the expression of hsp90 gene from the local isolate of Salmonella typhimuruim using RT-PCR. (Master's theses Theses and Dissertations Master). University of Baghdad. (2014).
https://search.emarefa.net/detail/BIM-601149
American Medical Association (AMA)
Khudayr, Marwah Muhsin. (2014). The effect of different temperatures on the expression of hsp90 gene from the local isolate of Salmonella typhimuruim using RT-PCR. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601149
Language
English
Data Type
Arab Theses
Record ID
BIM-601149
