Production of laccase from Pleurotus spp. by solid state fermentation using agricultural wastes
Other Title(s)
إنتاج اللاكييز من Pleurotus spp. بوساطة تخمرات الحالة الصلبة باستعمال فضلات زراعية
Dissertant
Thesis advisor
al-Azami, Abd al-Karim Jasim Hashim
Ali, Abd al-Jabbar Abbas
Comitee Members
Muhammad, Sanad B.
Khudayr, Sad Husayn
University
University of Baghdad
Faculty
College of Science
Department
Biotechnology Department
University Country
Iraq
Degree
Master
Degree Date
2013
English Abstract
The fungal isolates Pleurotus ostreatus and Pleurotus sapidus were evaluated for extracellular laccase production.
The two isolates were subjected to screening process to select the highest extracellular laccase production.
Results indicated that P.
ostreatus was showed the highest productivity of the enzyme.
Optimization studies were done by the solid state fermentation and revealed that the barley bran (7.5gm) was the best substrate, at initial moisture ratio 1:1.5 (w/v), using mineral salts solution as moisturizing solution with inoculums size 3 5 mm agar disk plugs at 25°C for 10 days to yield 2.93 U/mg for Laccase enzyme specific activity.
Extracellular laccase produced from Pleurotus ostreatus was purified by two steps including dialysis and DEAE- cellulose ion exchange chromatography.
The overall purification folds were 8.29 times with a 36.8% recovery.
The molecular weight of laccase was approximately 62.97 KDa as determined by SDS- PAGE.
The characterization of purified enzyme that the optimum pH for activity was 6.5 and (6.5-7.5) for stability.The maximum activity was observed at 45Cºand the enzyme showed 100% of its activity after 1h when incubated at temperature ranged (15-45) Cº, then the activity decreased with increasing temperature at 85Cº and the enzyme retained 4.41% of the initial activity, results also showed that enzyme retained 60% of its activity after 24h when incubated at optimum pH and temperature.
Laccase activity became 20% after 48 h of incubation.
The ability of P.
ostreatus culture to decolorize textile dyes revealed that the fungus grew well and completely decolorized Blue | P a g e 8 dye H3R with concentrations (50, 100, 150 and 200) and approximately 98% with 250 ppm concentration in comparison with yellow and Red dyes were very slowly processed, dye decolorization was appeared only at 50 ppm concentration.
The ability of partial purified laccase to decolorize of textile dyes showed that the blue H3R dye was completely decolorized in all concentrations within first min while yellow FG and red 3B dyes were decolorized in different percentage.
Main Subjects
Topics
No. of Pages
123
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Introduction.
Chapter One : Literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussions.
Conclusions and recommendations.
References.
American Psychological Association (APA)
Abd al-Rida, Shamam Sadi. (2013). Production of laccase from Pleurotus spp. by solid state fermentation using agricultural wastes. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601233
Modern Language Association (MLA)
Abd al-Rida, Shamam Sadi. Production of laccase from Pleurotus spp. by solid state fermentation using agricultural wastes. (Master's theses Theses and Dissertations Master). University of Baghdad. (2013).
https://search.emarefa.net/detail/BIM-601233
American Medical Association (AMA)
Abd al-Rida, Shamam Sadi. (2013). Production of laccase from Pleurotus spp. by solid state fermentation using agricultural wastes. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601233
Language
English
Data Type
Arab Theses
Record ID
BIM-601233
