Detection of respiratory syncytial virus infection in clinical samples using immunofluorescence test

Other Title(s)

الكشف عن الإصابة بفيروس Respiratory Syncytial Virus في العينات السريرية بواسطة اختبار التألق المناعي

Joint Authors

Awfi, Iman Mtshr
Ali, Layla Fuad
al-Suhayl, Raghad Ghalib

Source

Iraqi Journal of Science

Issue

Vol. 56, Issue 2C (30 Sep. 2015), pp.1612-1616, 5 p.

Publisher

University of Baghdad College of Science

Publication Date

2015-09-30

Country of Publication

Iraq

No. of Pages

5

Main Subjects

Biology
Medicine

Topics

Abstract AR

هدفت الدراسة الحالية للتحري عن الإصابة بفيروسRespiratory Syncytial Virus (RSV) في عينات من إفرازات الأنف و الحلق من المرضى الراقدين في المستشفى و الذين يعانون من أعراض عدوى الجهاز التنفسي بواسطة اختبار التألق المناعي.

في البداية تم اختبار هذه العينات لعدوى فيروس بواسطة reverse transcriptase - PCR.

العينات الموجبة تم فحصها باختبار التألق المناعي في خطوط خلايا Hep 2.

أظهرت النتائج أن العينات الموجبة بفحص RT-PCR كانت موجبة باختبار التألق المناعي.

هذه النتائج تبين أن اختبار التألق المناعي ذو حساسية عالية لتشخيص الإصابة بفيروس .RSV

Abstract EN

Current study aimed to investigate the Respiratory Syncytial Virus (RSV) in nasal secretion and throat swab samples of hospitalized patients with symptoms of respiratory tract infection using Immunofluorescence test.

Previously these samples were tested for Respiratory Syncytial Virus infection by reverse transcriptase-PCR assay.

The positive samples were tested by Immunofluorescence assay in monolayer confluent of Hep 2 cell line.

The results showed that the positive samples using the RT-PCR test were positive in this test.

These results reveal that Immunofluorescence test is sensitive method for detection the infection with RSV.

American Psychological Association (APA)

al-Suhayl, Raghad Ghalib& Ali, Layla Fuad& Awfi, Iman Mtshr. 2015. Detection of respiratory syncytial virus infection in clinical samples using immunofluorescence test. Iraqi Journal of Science،Vol. 56, no. 2C, pp.1612-1616.
https://search.emarefa.net/detail/BIM-606529

Modern Language Association (MLA)

al-Suhayl, Raghad Ghalib…[et al.]. Detection of respiratory syncytial virus infection in clinical samples using immunofluorescence test. Iraqi Journal of Science Vol. 56, no. 2C (2015), pp.1612-1616.
https://search.emarefa.net/detail/BIM-606529

American Medical Association (AMA)

al-Suhayl, Raghad Ghalib& Ali, Layla Fuad& Awfi, Iman Mtshr. Detection of respiratory syncytial virus infection in clinical samples using immunofluorescence test. Iraqi Journal of Science. 2015. Vol. 56, no. 2C, pp.1612-1616.
https://search.emarefa.net/detail/BIM-606529

Data Type

Journal Articles

Language

English

Notes

Text in English ; abstracts in English and Arabic.

Record ID

BIM-606529