Jestimation of efficient of random primers and effect of salt stress on chlorophyll B content in some bread wheat cultivars

Abstract EN

Random amplified polymorphic DNA (RAPD) analysis was employed to estimate genetic similarity among six bread wheat Triticum aestivum L.

genotypes profile changes in DIJLAH, IPPA99, FURAT, INIA66, URUQ and TAMOOZ 2.

Out of 12 random, 9 primers detected polymorphism among all the studied genotypes, produced 8-21 bands with an average of 14.5 bands per primer.

Wheat genotypes were grouped in three main groups, Group 1 was formed by 2 cultivars (DIJLAH and FURAT), group II was formed by three cultivars (INIA66, IPPA99 and URUQ), and group III was formed by a single cultivar (TAMOOZ 2) was the most distant genotype.

With three salinity levels the cultivars were distributed on main plots and the treatment were distributed on sub plots according to split – plot design.

Using three replication.

The cultivars (FURAT, URUQ and DIJLAH) in three salinity levels (0,5,10) EC (Elecetrical Conductivity) was able to prevent toxic levels of Na+, Cl- accumulation.

The cultivars (IPPA 99 and INIA 66) In 10 EC water salts build up in the intercellular space, leading to cell dehydration and death.

The highest chlorophyll content is recorded by Furat, while Ibaa99 is recorded the lowest chlorophyll content.

Salt stress led to decrease chlorophyll b content of leaves from (44.52 to 24.31) spad.