The bactericidal effect of CO2 laser on pseudomonas aeruginosa isolated from wound and burn infections, in-vitro
Other Title(s)
التأثير القاتل لليزر ثاني أوكسيد الكاربون على بكتريا الزوائف الزنجارية المعزولة من إصابات الجروح و الحروق خارج جسم الكائن الحي
Joint Authors
Ali, Aqilah Abd al-Zahrah
Hamzah, Bayda Fulayyih
Naji, Iman Natiq
Source
Issue
Vol. 12, Issue 3 (30 Sep. 2015), pp.485-495, 11 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2015-09-30
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract EN
A total of 60 cotton swabs are collected from patients suffering from burn wound and surgical site infections admitted to Baghdad Teaching Hospital and Burn Specialist Hospital in Baghdad city during 9/2013 to 11/2013.
All cotton swabs are cultured initially on blood agar and MacConkey agar and subjected for standard bacteriological procedures for bacteriological diagnosis.
Twenty samples out of sixty are identified as Pseudomonas aeruginosa by conventional methods.
The results of antibiotic susceptibility test illustrate that the antibiotics resistance rate of Pseudomonas aeruginosa isolates is as follows:100% (20\20) for ceftriaxone, cefepime and carbencillin, 70% (14/20) for amikacin, 65%(13/20) for tobramycin, ceftazidim and gentamycin, 55% (11/20) for ciprofloxacin and norfloxacin, 50% (10/20) for piperacillin and impeneme, 30% (6/20) for aztreonam.
All Pseudomonas aeruginosa isolates are investigated for detection of some virulence factors (haemolysin, protease, lipase enzymes, and extracellular pigments) and biofilm formation.
The results of virulence factors reveal that all the isolates are haemolysin, protease, lipase enzymes and extracellular pigments producer, while 95% of the isolates are biofilm producer.
Six isolates are selected to irradiation by using CO2 laser according to the results of antibiotic susceptibility and virulence factors at power densities (2000, 2500, and 3000) W/cm2 with exposure time (60 and 90) second.
The results of CO2 laser irradiation illustrate that CO2 laser irradiation lead to a reduction in the mean value of the viable number CFU/ml of Pseudomonas aeruginosa isolates with the increase of the power density and exposure time.
The results of the statistical analysis by using analysis of variance (ANOVA) one way and least significant differences-LSD show that there are statistical significant differences in the mean of the viable number CFU/ml between different power densities and different exposure times.
After irradiation, antibiotic susceptibility and virulence factors tests of the irradiated strains are performed.
The current study concludes that CO2 laser has bactericidal effect on P.
aeruginosa isolates without any effect on its antibiotics susceptibility and virulence factors.
American Psychological Association (APA)
Naji, Iman Natiq& Ali, Aqilah Abd al-Zahrah& Hamzah, Bayda Fulayyih. 2015. The bactericidal effect of CO2 laser on pseudomonas aeruginosa isolated from wound and burn infections, in-vitro. Baghdad Science Journal،Vol. 12, no. 3, pp.485-495.
https://search.emarefa.net/detail/BIM-609165
Modern Language Association (MLA)
Naji, Iman Natiq…[et al.]. The bactericidal effect of CO2 laser on pseudomonas aeruginosa isolated from wound and burn infections, in-vitro. Baghdad Science Journal Vol. 12, no. 3 (2015), pp.485-495.
https://search.emarefa.net/detail/BIM-609165
American Medical Association (AMA)
Naji, Iman Natiq& Ali, Aqilah Abd al-Zahrah& Hamzah, Bayda Fulayyih. The bactericidal effect of CO2 laser on pseudomonas aeruginosa isolated from wound and burn infections, in-vitro. Baghdad Science Journal. 2015. Vol. 12, no. 3, pp.485-495.
https://search.emarefa.net/detail/BIM-609165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 492-494
Record ID
BIM-609165