An in vitro histological study of human skin wound soldering using 980 nm diode laser in continuous versus pulsed modes

Abstract EN

-Background: Laser skin wound soldering offers many distinct advantages over conventional closure and laser welding techniques.

Objective : to compare the histological effects of human skin wound soldering using 50 % human albumin solder and compound charcoal photosensitiser with 980 nm diode laser acting in various modes of action and parameters.

Study Design/Materials and Methods: In this in vitro experimental study , Multiple 3-4 cm long full thickness incisions in a specimen of human skin were soldered using a 4 mm spot diameter beam of 980 nm diode laser(at different laser parameters and modes of action) with 50 % human albumin solder mixed with the compound charcoal at 5 % W/V concentration .After obtaining a successful wound soldering , the wound edge were excised and then studied histologically.

Results: Although a single pulse per shot , spot by spot soldering technique has resulted in a weaker soldering of the wound experimentally, yet it was less tissue harmful than the continuous mode laser, inspite that the later had resulted in a stronger wound soldering.

A strong wound closure didn't happen using repetitive pulsed laser mode at any of the tested parameters or action modes.

Conclusion: A single spot by spot , pulsed 980 nm laser wound soldering is less tissue harmful than the continuous mode laser soldering although it has resulted in a weaker soldered wound initial tensile strength