Histological evaluation of local application of collagen I and-or vascular endothelial growth factor : an experimental study in rats
Joint Authors
Rashid, Rafal
al-Hijazi, Adhra Yahya
Source
Journal of Baghdad College of Dentistry
Issue
Vol. 27, Issue 3 (30 Sep. 2015), pp.79-84, 6 p.
Publisher
University of Baghdad College of Dentistry
Publication Date
2015-09-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
Background : The study was designed to evaluate the effect of local application of exogenous VEGF / collagen I separately and as a combination in socket healing.
Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1st molar of both sides (right side was considered as experimental site, while left be the control one, treated with 1μL of normal saline).
The rats were scarified at 3, 7, 14, 28 days post extraction.
Socket healing was histologically examined with immunohistochemistochemical localization of ALP&FGF2.
Materials and Method : Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1stmolar of both sides (right side was considered as experimental site, while left be the control one).
The animals were divided into following groups according to the applicable of biomaterials.
A.
Control group the tooth socket treated with 1μL of normal saline B.
Experimental group includes • Group I contains (20) rats, the tooth socket treated with 1μL of VEGF • Group II contains (20) rats the tooth socket treated with 1μL of collagen type I • Group III contains (20) rats, the tooth socket treated with 1μL of a combination of VEGF and collagen I.
Results: At 28 days all groups show re-epithelization but in different thickness, and with newly bone apposition and with different maturity.
For positive cells expressed ALP, VEGF group records a high mean values at 3, 14, 28 days periods and with high differences in comparison to other groups while control group reports a high mean value at 7 days.
For positive cells expressed FGF2.
Control group illustrates a high record for the mean of positive cells expressed FGF2 at 3,7days periods and with high differences in comparison to other groups, while combination group reports a high mean value at 14 days .
Conclusion: Results, high lighted on the effect of local application of VEGF in extracted tooth socket that facilitate epithelization, while combination of (collagen and VEGF) shows a high mineralization zone.
American Psychological Association (APA)
Rashid, Rafal& al-Hijazi, Adhra Yahya. 2015. Histological evaluation of local application of collagen I and-or vascular endothelial growth factor : an experimental study in rats. Journal of Baghdad College of Dentistry،Vol. 27, no. 3, pp.79-84.
https://search.emarefa.net/detail/BIM-611503
Modern Language Association (MLA)
Rashid, Rafal& al-Hijazi, Adhra Yahya. Histological evaluation of local application of collagen I and-or vascular endothelial growth factor : an experimental study in rats. Journal of Baghdad College of Dentistry Vol. 27, no. 3 (Sep. 2015), pp.79-84.
https://search.emarefa.net/detail/BIM-611503
American Medical Association (AMA)
Rashid, Rafal& al-Hijazi, Adhra Yahya. Histological evaluation of local application of collagen I and-or vascular endothelial growth factor : an experimental study in rats. Journal of Baghdad College of Dentistry. 2015. Vol. 27, no. 3, pp.79-84.
https://search.emarefa.net/detail/BIM-611503
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 84
Record ID
BIM-611503