Spectrophotometric determination of trimethoprim using 2,4-dinitro-1-fluorobenzene reagent
Joint Authors
al-Subhah, Diya Najm
Hammudi, Intisar A.
Source
al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah
Issue
Vol. 24, Issue 2 (30 Jun. 2011), pp.1-12, 12 p.
Publisher
University of Mosul College of Education for Pure Science
Publication Date
2011-06-30
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract EN
A simple spectrophotometric method for the determination of trimethoprim (TMP) in pure form and in its pharmaceutical formulation has been described.
The method is based on the reaction of TMP with 2,4-dinitro-1-fluorobenzene reagent (DNFB) in acetone medium to give a highly rosy red colored product with maximum absorption at 538 nm with a molar ratio of 1:1.
Beer's law is obeyed in the range 10-75 μg/ml with molar absorptivity of 1.917x103 l.mol-1.cm-1 and Sandell's sensitivity of 0.1514 μg/cm2.
Precision (RSD) better than 1.7 % and accuracy (average recovery %) is 100.76 %.
The suggested method has been applied to dosage forms as tablets and compared with the pharmacopoeial method, t-test is evaluated and found 1.828 at 95% confidence limit.
The results show that there is no interference are present in commercial dosage forms.
American Psychological Association (APA)
al-Subhah, Diya Najm& Hammudi, Intisar A.. 2011. Spectrophotometric determination of trimethoprim using 2,4-dinitro-1-fluorobenzene reagent. al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah،Vol. 24, no. 2, pp.1-12.
https://search.emarefa.net/detail/BIM-620827
Modern Language Association (MLA)
al-Subhah, Diya Najm& Hammudi, Intisar A.. Spectrophotometric determination of trimethoprim using 2,4-dinitro-1-fluorobenzene reagent. al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah Vol. 24, no. 2 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-620827
American Medical Association (AMA)
al-Subhah, Diya Najm& Hammudi, Intisar A.. Spectrophotometric determination of trimethoprim using 2,4-dinitro-1-fluorobenzene reagent. al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah. 2011. Vol. 24, no. 2, pp.1-12.
https://search.emarefa.net/detail/BIM-620827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 10-12
Record ID
BIM-620827