Detection of RAF fusion transcripts in FFPE samples of medullablastoma and ependymom in Iraqi children with RT-RQPCR assays
Other Title(s)
التحري عن نسخ ملتحمات مورث RAF في عينات مثبتة بشمع البرافينين لأورام الأرومة النخاعية أاورام ارومة االبطانة العصبية في الأطفال العراقيين باختبار
Joint Authors
al-Ubydi, Lama Hasan Ulwan
al-Ansary, Nada Abd al-Majid
Muhammad, Haydar Latif
al-Rikabi, Nahi Yusuf Yasin
Tubal, Khalid Abd al-Hamzah
Source
Issue
Vol. 11, Issue 3(s) (31 Dec. 2014), pp.1301-1309, 9 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2014-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract AR
Medulloblastomas and ependymomas are the most common malignant brain tumors in children.
However genetic abnormalities associated with their development and prognosis remain unclear.
Recently two gene fusions, KIAA1549–BRAF and SRGAP3–RAF1 have been detected in a number of brain tumours.
We report here our development and validation of RT-RQPCR assays to detect various isoforms of these two fusion genes in formalin fixed paraffin embedded (FFPE) tissues of medulloblastoma and ependymoma.
We examined these fusion genes in 44 paediatric brain tumours, 33 medulloblastomas and 11 ependymomas.
We detected both fusion transcripts in 8/33, 5/33 SRGAP3 ex10/RAF1 ex10, and 3/33 KIAA1549 ex16/BRAF ex9, meduloblastomas but none in the 11 ependymomas examined.
This investigation provided evidence to the value of RT-RQPCR assays for the detection of these fusion genes in large-scale studies on FFPE tissues.
The study also reports the first detection of RAF fusion genes in meduloblstomas.
Abstract EN
Medulloblastomas and ependymomas are the most common malignant brain tumors in children.
However genetic abnormalities associated with their development and prognosis remain unclear.
Recently two gene fusions, KIAA1549–BRAF and SRGAP3–RAF1 have been detected in a number of brain tumours.
We report here our development and validation of RT-RQPCR assays to detect various isoforms of these two fusion genes in formalin fixed paraffin embedded (FFPE) tissues of medulloblastoma and ependymoma.
We examined these fusion genes in 44 paediatric brain tumours, 33 medulloblastomas and 11 ependymomas.
We detected both fusion transcripts in 8/33, 5/33 SRGAP3 ex10/RAF1 ex10, and 3/33 KIAA1549 ex16/BRAF ex9, meduloblastomas but none in the 11 ependymomas examined.
This investigation provided evidence to the value of RT-RQPCR assays for the detection of these fusion genes in large-scale studies on FFPE tissues.
The study also reports the first detection of RAF fusion genes in meduloblstomas.
American Psychological Association (APA)
al-Ubydi, Lama Hasan Ulwan& al-Ansary, Nada Abd al-Majid& al-Rikabi, Nahi Yusuf Yasin& Muhammad, Haydar Latif& Tubal, Khalid Abd al-Hamzah. 2014. Detection of RAF fusion transcripts in FFPE samples of medullablastoma and ependymom in Iraqi children with RT-RQPCR assays. Baghdad Science Journal،Vol. 11, no. 3(s), pp.1301-1309.
https://search.emarefa.net/detail/BIM-622024
Modern Language Association (MLA)
al-Ubydi, Lama Hasan Ulwan…[et al.]. Detection of RAF fusion transcripts in FFPE samples of medullablastoma and ependymom in Iraqi children with RT-RQPCR assays. Baghdad Science Journal Vol. 11, no. 3 (2014), pp.1301-1309.
https://search.emarefa.net/detail/BIM-622024
American Medical Association (AMA)
al-Ubydi, Lama Hasan Ulwan& al-Ansary, Nada Abd al-Majid& al-Rikabi, Nahi Yusuf Yasin& Muhammad, Haydar Latif& Tubal, Khalid Abd al-Hamzah. Detection of RAF fusion transcripts in FFPE samples of medullablastoma and ependymom in Iraqi children with RT-RQPCR assays. Baghdad Science Journal. 2014. Vol. 11, no. 3(s), pp.1301-1309.
https://search.emarefa.net/detail/BIM-622024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references. 1415-1418
Record ID
BIM-622024
