Biochemical supplementation of excess dietary biotin in chickens

Abstract EN

The present study was designed to investigate the possible effects of excess dietary biotin supplementation on electrophoretic pattern of serum proteins and the level of serum free amino acids and some biochemical parameters which reflect changes of carbohydrates and lipids metabolism in the blood of Hubbard chicks.

A total number of 150 one-day-old Hubbard chicks were divided into two main groups.

The control group reared on a basal chicken rations while the second group fed on a basal diet supplemented with biotin, where biotin was added as 0.2mg / kg body weight, (double dietary daily-requirement) for 8 weeks.

Blood for serum samples collected from each group at 4 and 8 weeks of supplementation, the separated serum was used directly for the subsequent biochemical analysis.

The data revealed a significant increases (P<0.05) in serum total protein, albumin and total globulin’s (alpha, beta and gamma globulin) levels at 4 weeks of experiment; this increase became highly significant (P<0.01) after 8 weeks.

A significant (P<0.05) decrease recorded in serum total lipids, total-cholesterol, triacylglycerol and phospholipids concentrations at 8 weeks.

On the other hand a significant increase (P<0.05) in serum glucose, T3 and T4 levels at 8 weeks.

While there was a significant decrease was recorded (P<0.05) in the concentration of both muscle and liver glycogen at 4 and 8 weeks.

However there was no significant changes in the mean values of amino acid patterns during the experimental periods.

From the present study we conclude that supplementation of biotin very important for chickens because biotin is a cofactor in carboxylation and decarboxyl-ation reactions involving fixation of CO2.

Also affect in carbohydrates, lipids and proteins metabolism.