Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of streptomyces sp. strain MJM 8637
Joint Authors
Lee, Sung Kwon
Lee, Dong Ryung
Choi, Bong Keun
Palaniyandi, Sasikumar Arunachalam
Yang, Seung Hwan
Suh, Joo-Won
Source
Saudi Journal of Biological Sciences
Issue
Vol. 22, Issue 6 (30 Nov. 2015), pp.744-751, 8 p.
Publisher
Publication Date
2015-11-30
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
Abstract EN
To investigate the anti-cancer properties of soil-borne actinobacteria, MJM 8637, the glutathione S-transferase pi (GST-pi) assay, anti-tumor necrosis factor (TNF)-a assay, the level of antioxidant potential by DPPH radical scavenging activity, NO scavenging activity, and ABTS radical scavenging activity in ethyl acetate extract were determined.
The 16S rDNA sequencing analysis revealed that Streptomyces sp.
strain MJM 8637, which was isolated from Hambak Mountain, Korea, has 99.5% similarity to Streptomyces atratus strain NBRC 3897.
The physiological and the morphological characteristics of the strain MJM 8637 were also identified.
The ethyl acetate extract of MJM 8637 inhibited TNF-a production approximately 61.8% at concentration 100 lg/ml.
The IC50 value of the strain MJM 8637 extract on GST-pi was identified to be 120.2 ± 1.6 lg/ml.
In DPPH, NO, and ABTS radical scavenging assays, the IC50 values of the strain MJM 8637 extract were found to be 977.2 lg / ml, 1143.7 lg / ml, and 454.4 lg / ml, respectively.
The ethyl acetate extract of the strain MJM 8637 showed 97.2 ±1.3% of cell viability at 100 lg/ml in RAW 264.7 cell viability assay.
The results obtained from this study suggest that the ethyl acetate extract of Streptomyces sp.
strain MJM 8637 could be considered as a potential source of drug for the cancers that have multidrug resistance with its GST-pi inhibition and anti-inflammation activities, and low cytotoxicity.
American Psychological Association (APA)
Lee, Sung Kwon& Lee, Dong Ryung& Choi, Bong Keun& Palaniyandi, Sasikumar Arunachalam& Yang, Seung Hwan& Suh, Joo-Won. 2015. Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of streptomyces sp. strain MJM 8637. Saudi Journal of Biological Sciences،Vol. 22, no. 6, pp.744-751.
https://search.emarefa.net/detail/BIM-628756
Modern Language Association (MLA)
Lee, Sung Kwon…[et al.]. Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of streptomyces sp. strain MJM 8637. Saudi Journal of Biological Sciences Vol. 22, no. 6 (Nov. 2015), pp.744-751.
https://search.emarefa.net/detail/BIM-628756
American Medical Association (AMA)
Lee, Sung Kwon& Lee, Dong Ryung& Choi, Bong Keun& Palaniyandi, Sasikumar Arunachalam& Yang, Seung Hwan& Suh, Joo-Won. Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of streptomyces sp. strain MJM 8637. Saudi Journal of Biological Sciences. 2015. Vol. 22, no. 6, pp.744-751.
https://search.emarefa.net/detail/BIM-628756
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 750-751
Record ID
BIM-628756