Using the anchored microsatellite primed polymerase chain reaction (amp-PCR)‎ technique to study genetic diversity in four local tomato (lycopersicum esculentum)‎ cultivars in Palestine

Abstract EN

Although there are only four local cultivars of tomato in Palestine, the genetic variation among these cultivars has not been studied yet.

The genetic variation among the four local cultivars and their regenerant plants was determined using anchored Inter Simple Sequence Repeat (ISSR) regions as a preliminary step towards developing a tomato cultivar resistant to abiotic and biotic stresses through gene transfer and molecular marker-assisted breeding,.

Ten 3'-anchored semi-specific primers were employed.

The results showed that the least similarity exists between cultivars H3 and P1 (74%) while the highest is between cultivars B2 and R4 (93.3%).

These results demonstrate the existence of genetic variation among the local cultivars, which is an important step for the ongoing breeding and selection programs.