Characterization of a multi-resistant bacterial strain isolated from a Moroccan patient suffering from urinary infection : identification and molecular analysis of a related mega-plasmid harboring antibiotic resistance genes
Dissertant
Thesis advisor
Sindidi, Khalid Sadaqa
Anissi, Jawad
Comitee Members
Berrada, Fuad
Remmal, Adnani
al-Asli, Abd al-Ghani
University
Al Akhawayn University
Faculty
School of Science and Engineering
Department
Biotechnology
University Country
Morocco
Degree
Master
Degree Date
2011
English Abstract
Gram-negative bacteria producing VIM-type metallo--lactamases (MBLs) are increasingly isolated worldwide.
A strain called Adn01 was isolated and phenotypic tests attributed this strain to the genus Serratia.
Combined disc tests using carbapenems with and without EDTA on the same plate demonstrated the production of metallo--lactamase (MBL).
This strain harbors a mega-plasmid mediated antibiotic resistance genes that demonstrated its transferability to an E.
coli competent strain.
E.
coli transformants become resistant to some antibiotics as do the wild type in comparison to the non transformant E.
coli.
Integrons sequences were used to design primers sets.
Standard polymerase chain reaction (PCR) conditions were used to amplify several antibiotic resistance genes belonging to the KPC family of nonmetallo carbapenemases class A (NMC-A); class B metallo beta lactamase enzyme of VIM family and a beta lactamase gene of class C cephalosporinases (AmpC) coding for carbapenemases, extended-spectrum -lactamases (ESBLs).
Demonstration of such resistance genes related to VIM-type metallo--lactamases (MBLs) contained in a mega-plasmid from the isolated Adn01 strain is demonstrated in the first time in this study.
Main Subjects
Topics
No. of Pages
102
Table of Contents
Table of contents.
Abstract.
Abstract in French.
Chapter One : General overview.
Chapter Two : Introduction.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
Chapter Five : General conclusion.
References.
American Psychological Association (APA)
Aragosa, Aminah. (2011). Characterization of a multi-resistant bacterial strain isolated from a Moroccan patient suffering from urinary infection : identification and molecular analysis of a related mega-plasmid harboring antibiotic resistance genes. (Master's theses Theses and Dissertations Master). Al Akhawayn University, Morocco
https://search.emarefa.net/detail/BIM-629700
Modern Language Association (MLA)
Aragosa, Aminah. Characterization of a multi-resistant bacterial strain isolated from a Moroccan patient suffering from urinary infection : identification and molecular analysis of a related mega-plasmid harboring antibiotic resistance genes. (Master's theses Theses and Dissertations Master). Al Akhawayn University. (2011).
https://search.emarefa.net/detail/BIM-629700
American Medical Association (AMA)
Aragosa, Aminah. (2011). Characterization of a multi-resistant bacterial strain isolated from a Moroccan patient suffering from urinary infection : identification and molecular analysis of a related mega-plasmid harboring antibiotic resistance genes. (Master's theses Theses and Dissertations Master). Al Akhawayn University, Morocco
https://search.emarefa.net/detail/BIM-629700
Language
English
Data Type
Arab Theses
Record ID
BIM-629700
