Association of virulent genes hly, sfa, cnf-1 and pap with antibiotic sensitivity in escherichia coli strains isolated from children with community-acquired UTI
Joint Authors
Japoni, A.
Emamghoraishi, F.
Farshad, S.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 12, Issue 1 (30 Oct. 2010), pp.33-37, 5 p.
Publisher
Publication Date
2010-10-30
Country of Publication
United Arab Emirates
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background : recent studies have suggested that the decrease in the pathogenicity of E.
coli is due to acquisition of resistance to some antibiotics.
This study was performed to investigate four virulence factors of pap, cnf-1, sfa and hly in resistant E.
coli and compare them with susceptible strains of the bacteria isolated from children with community–acquired UTI.
Methods : drug sensitivity of 96 E.
coli isolates was evaluated using a disc diffusion method.
The prevalence of virulence genes was determined by PCR.
Results : E.
coli strains showed a high degree of sensitivity to impanel, amikacin, nitrofurantoin and ciprofloxacin.
Approximately, 80.2% of the isolates were resistant to ampicillin.
Only 12.5% of the strains were susceptible to all tested antibiotics.
PCR showed that cnf-1 (22.9%) was more prevalent than hly (15.6%) and among adhesion coding genes; pap (30.2%) was more prevalent than sfa (18.8%).
In all strains, the expression of all virulent genes was less prevalent in most antibiotic resistant groups than in susceptible ones but not statistically significant except for genotypes of pap + - cnf +, pap + - hly + and cnf + - hly + with nalidixic acid.
Conclusion : we propose that pap and cnf-1 genes in combination with hly gene constitute an uropathogenic genomic configuration which is the characteristic of the nalidixic-acid susceptible E.
coli strains, causing urinary tract infection.
American Psychological Association (APA)
Farshad, S.& Emamghoraishi, F.& Japoni, A.. 2010. Association of virulent genes hly, sfa, cnf-1 and pap with antibiotic sensitivity in escherichia coli strains isolated from children with community-acquired UTI. Iranian Red Crescent Medical Journal،Vol. 12, no. 1, pp.33-37.
https://search.emarefa.net/detail/BIM-63036
Modern Language Association (MLA)
Farshad, S.…[et al.]. Association of virulent genes hly, sfa, cnf-1 and pap with antibiotic sensitivity in escherichia coli strains isolated from children with community-acquired UTI. Iranian Red Crescent Medical Journal Vol. 12, no. 1 (Oct. 2010), pp.33-37.
https://search.emarefa.net/detail/BIM-63036
American Medical Association (AMA)
Farshad, S.& Emamghoraishi, F.& Japoni, A.. Association of virulent genes hly, sfa, cnf-1 and pap with antibiotic sensitivity in escherichia coli strains isolated from children with community-acquired UTI. Iranian Red Crescent Medical Journal. 2010. Vol. 12, no. 1, pp.33-37.
https://search.emarefa.net/detail/BIM-63036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 37
Record ID
BIM-63036