Extraction and characterization of a chromosomal stain from black mulberry (morus nigra)‎

Abstract EN

A dark-blue chromosomal stain from Black Mulberry (Morus nigra) was extracted using organic solvents including dioxane, n-hexane, petroleum ether and acetone.

This extracted stain stained chromosomes satisfactory.

In order to ascertain purity of the extracted stain, it was compared spectrophotometery with three commercial standards stains; they are Azur II Eosin, Giemsa’s and Methylene blue Stains using a 200-900nm Spectrophotometric scan at three different concentrations (25, 50 and 100mg / ml). It was found that the second peak produced by Azur II eosin and Giemsa stain (as well as the single peak produces by Methylene blue) occupied the same area occupied by the second peak of the extracted stain, which agree to some degree of similarity.

The extracted method proved to be an appropriate analytical technique with hope that further trials can be carrying out to simplify the extracting of the stain in future researches and make it less expensive. Keywords: chromosomal stain, extraction, Mullberry.