Hsp70-2 polymorphism in urinary bladder carcinoma

Abstract EN

hsp70-2 or (hspa1b) is one of the human HSP70 genes, it was originally found specifically in primary spermatocytes and spermatids, that played an important role in spermatogenesis.

Now HSP70-2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues.

HSP70-2 has been identified as a potential cancer-promoting protein expressed at abnormal levels in a subset of human cancers such as bladder urothelial cancer.

So, the present study aimed to study the HSP70-2 polymorphism in 50 blood sample of patients with urinary bladder carcinoma (UBC) and 15 healthy subjects, also study HSP70-2 gene expression in 40 paraffin tissue sections of patients with urinary bladder carcinoma UBC and 10 paraffin tissue section in patients with urinary bladder diseases UBD.

A PCR-RFLP test was used to study the HSP70-2 gene polymorphism.

r RT-PCR was used to study HSP70-2 gene expression.

Genotype distribution and allele frequencies of HSP70-2 polymorphisms showed HSP70-2 P1P2 genotype frequency and HSP70-2 P2 allele were significantly increased in the patients with UBC compare to healthy subjects(p≤0.01).Also, the expression mean of HSP70-2 gene is 39.4 fold higher in UBC than in UBD patients.