Detection of human rhinovirus (HRV) in upper respiratory tract infections by RT-PCR technique
Joint Authors
Dakhil, Qasim Raysan
Nasir, Hassan Hashim
Sayhud, Anwar Salih
Source
Issue
Vol. 12, Issue 3 (30 Sep. 2015), pp.576-580, 5 p.
Publisher
University of Babylon College of Medicine
Publication Date
2015-09-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
Human rhinovirus (HRV) is the most common respiratory microorganism, which causes acute respiratory infections especially in healthy young children, elderly people, and the immunocompromised person.
The diagnosis of rhinovirus infection by clinical and convenient means is more complicated because of the lack of sensitive, accurate tools for differentiation of the rhinovirus from other respiratory tract viral infections.
Here, we had developed a rapid, specific and sensitive one step Reverse Transcription Real-Time PCR molecular method for the detection of human rhinovirus (HRV) from nasopharyngeal secretion specimens.
The Primers designed from the highly conserved polyprotein gene of human rhinovirus.
Our results that had shown a positive RT-PCR assay were specific in detection of HRV and observed in 41 out of 50 specimens (82%).
We conclude that onestep RT-PCR assay was a highly specific and rapid technique for the detection of HRV RNA in nasopharyngeal secretions of patients with acute respiratory tract infections.
American Psychological Association (APA)
Dakhil, Qasim Raysan& Sayhud, Anwar Salih& Nasir, Hassan Hashim. 2015. Detection of human rhinovirus (HRV) in upper respiratory tract infections by RT-PCR technique. Medical Journal of Babylon،Vol. 12, no. 3, pp.576-580.
https://search.emarefa.net/detail/BIM-644669
Modern Language Association (MLA)
Dakhil, Qasim Raysan…[et al.]. Detection of human rhinovirus (HRV) in upper respiratory tract infections by RT-PCR technique. Medical Journal of Babylon Vol. 12, no. 3 (2015), pp.576-580.
https://search.emarefa.net/detail/BIM-644669
American Medical Association (AMA)
Dakhil, Qasim Raysan& Sayhud, Anwar Salih& Nasir, Hassan Hashim. Detection of human rhinovirus (HRV) in upper respiratory tract infections by RT-PCR technique. Medical Journal of Babylon. 2015. Vol. 12, no. 3, pp.576-580.
https://search.emarefa.net/detail/BIM-644669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 579-580
Record ID
BIM-644669