Extraction, partial purification and characterization of inulinase produced from Aspergillus Niger AN20 by solid state fermentation
Joint Authors
Aziz, Ghazi M.
Abd al-Amir, Zaynab Walid
al-Sayyid, Ali J. R.
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 2 (31 Dec. 2015), pp.111-122, 12 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
Inulinase produced by Aspergillus niger AN20 was partially purified using two chroma-tographic techniques, ion exchange chromatography by DEAE-cellulose and gel filtration by Se-phadex G-150.
The two steps gave specific activity of 810 U/mg proteins, the purification fold was 8.1 and enzymatic yield was 33.5 %.
Some biochemical characteristics of the partially purified in-ulinase were determined and the results revealed that the enzyme have a molecular weight of 42 KDa., optimum pH for inulinase activity was 4.5, and the pH of the enzyme stability was the range 4.0-8.0.
The maximum activity of purified inulinase activity from A.
niger AN20 was determined as 50 ᴼC, while the thermal stability ranges from 20-50 Cᵒ.
The aim of this study was the extraction and purification of inulinase produced from Aspergillus niger AN20 by solid state fermentation.
American Psychological Association (APA)
Aziz, Ghazi M.& Abd al-Amir, Zaynab Walid& al-Sayyid, Ali J. R.. 2015. Extraction, partial purification and characterization of inulinase produced from Aspergillus Niger AN20 by solid state fermentation. Iraqi Journal of Biotechnology،Vol. 14, no. 2, pp.111-122.
https://search.emarefa.net/detail/BIM-644818
Modern Language Association (MLA)
Aziz, Ghazi M.…[et al.]. Extraction, partial purification and characterization of inulinase produced from Aspergillus Niger AN20 by solid state fermentation. Iraqi Journal of Biotechnology Vol. 14, no. 2 (2015), pp.111-122.
https://search.emarefa.net/detail/BIM-644818
American Medical Association (AMA)
Aziz, Ghazi M.& Abd al-Amir, Zaynab Walid& al-Sayyid, Ali J. R.. Extraction, partial purification and characterization of inulinase produced from Aspergillus Niger AN20 by solid state fermentation. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 2, pp.111-122.
https://search.emarefa.net/detail/BIM-644818
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 121-122
Record ID
BIM-644818
