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Molecular diagnosis of Lactobacillus microbiota in some healthy Iraqi women
Author
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 2 (31 Dec. 2015), pp.123-134, 12 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract EN
Molecular diagnosis of Lactobacillus species resident in vaginal ecosystem in some healthy women of Baghdad city was investigated using 16S-23SInternal Transcribed Spacer region (ITS-1) in multiplex PCR reactions with two stages.
The first stage used for grouping of Lactobacilli, followed by second stage multiplex reactions.
Different PCR products derived from 35 vaginal swaps were obtained, 10 of these products were chosen randomly and subjected for sequencing.
Sequences alignment and in silico diagnosis using BLAST against nr databases, showed that Lactobacillus crispatus comprised the majority (40 %),followed by Lb.
gasseri (30 %), the rest Lb.
jensenii, Lb.
acidophilus were 10% for each.
It was noticed that Lb.
crispatus and Lb.
gasseri showed a little variation which is expected due to inter individual and environment variation.
American Psychological Association (APA)
Rashid, Marib Nazih. 2015. Molecular diagnosis of Lactobacillus microbiota in some healthy Iraqi women. Iraqi Journal of Biotechnology،Vol. 14, no. 2, pp.123-134.
https://search.emarefa.net/detail/BIM-644824
Modern Language Association (MLA)
Rashid, Marib Nazih. Molecular diagnosis of Lactobacillus microbiota in some healthy Iraqi women. Iraqi Journal of Biotechnology Vol. 14, no. 2 (2015), pp.123-134.
https://search.emarefa.net/detail/BIM-644824
American Medical Association (AMA)
Rashid, Marib Nazih. Molecular diagnosis of Lactobacillus microbiota in some healthy Iraqi women. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 2, pp.123-134.
https://search.emarefa.net/detail/BIM-644824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 131-134
Record ID
BIM-644824