الكشف عن السم Diacetoxyscirpenol في حبوب الذرة الصفراء في العراق بتقنية صفائح الكروماتوكرافي الرقيقة

Abstract EN

This study was conducted to detect Diacetoxyscirpenol (DAS) mycotoxin produced by Fusarium spp in corn seeds and to detect some fungal isolates that produce it .The detection of mycotoxin was archived by Thin Layer Chromotography.

Corn seeds collected from some area and tow isolates of F.graminearum and F.culmorum were got from ministry of science and technology to use them of DAS production .

A part of corn sampeles was used directly to extract and detect DAS toxin by TLC while the other part were used for isolation of Fusarium spp.

Results showed that10 isolates from 15 of Fusarium spp produced DAS toxin , some of species was F.graminearum and F.culmorum also the result of directed detection of DAS mycotoxin showed that DAS associated with most samples and 90.4% of corn seed samples contained DAS toxin for the first time in Iraq , The quantitative detection on DAS mycotoxin by gas liquid chromatography showed deference of Fusarium spp.

isolates in DAS production according to site of isolate where is the college of agriculture isolate was the more active in DAS production with 653.89 ng/g , the second isolate in DAS production was Al-Hilla isolate that produced 811884 ng/g from DAS toxin while AL-Rhadwania isolate was 206.34 ng/g and then AL-Basrah isolate that belong to F.graminearum with 65.23 ng/g.

This study is the first of recording of those species to secreting DAS toxin in Iraq .We concluded that DAS founded in a high amounts on corn seed in Iraq .We recommend use of suitable storing environment to prevent growth of fungi that producing toxins.