In vitro production of interleukin 27 and Its immunoregulatory function upon CD4 differentiation into Th17 and Th1
Dissertant
Thesis advisor
Ibrahimi, Izz al-Din
Gauchat, Jean Francois
Comitee Members
al-Asli, Abd al-Ghani
Barradah, Fuad
Meddah, Bushra
Khalid, Sendide
University
Al Akhawayn University
Faculty
School of Science and Engineering
Department
Biotechnology
University Country
Morocco
Degree
Master
Degree Date
2010
English Abstract
The etiopathology of autoimmune diseases remains elusive.
Accumulating evidences suggest that T cell differentiation into T helper cells plays an important role in the overactive immune response.
T helper responses have now grown to include three T cell subsets: Th1, Th2 and Th17.
Th17 cells have recently emerged as a third independent T cell subset which may play an essential role in protection against certain extra-cellular pathogens.
However, Th17 cells with specificity for self antigens are highly pathogenic and lead to the development of inflammation and severe autoimmunity.
A combination of TGF-β and IL-6 was recently described to be essential for initial differentiation of Th17 cells.
Interestingly, IL-12 family member IL-27 seems to play a key role in the inhibition of Th17 differentiation.
IL-27 is the newest member of the IL-12 family heterodimeric cytokines composed of Epstein Barr virus induced gene 3 (EBI3) and p28 chains and an early product of activated macrophages and dendritic cells.
IL-27doesn’t only play an important role in the regulation of differentiation of naive T helper cells but also possesses anti-inflammatory properties.
In this study, we produced the recombinant protein EBI3 by molecular biology tools, purified CD4+ T cells from female (C57/BL) mice monocytes and investigated whether we can success in producing IL-27 in vitro by adding an excess of EBI3 to CD4+ T cells knowing that p28 subunit is produced after 8 hours of cellular treatment with different cytokines conditions.
Using flowcytometry, we observed that IL-27 produced in vitro inhibited the differentiation of Th17 while it activated Th1 polarisation.
As a next step, we wanted to test the cytokine IL-27 in vivo.
We then aimed to create a chimeric cDNA encoding for EBI3 fused to a mouse immunoglobulin G1 (IgG1) Fc region.
The benefit behind this work is to test whether the combination of EBI3 and p28 in vitro and in vivo could decrease the severity of inflammation and therefore be a new strategy for autoimmune disorders therapy
Main Subjects
Mathematics
Information Technology and Computer Science
Topics
No. of Pages
57
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Materials and methods.
Chapter Three : Results.
Chapter Four : Conclusion and perspectives.
References.
American Psychological Association (APA)
Chehboun, Salma. (2010). In vitro production of interleukin 27 and Its immunoregulatory function upon CD4 differentiation into Th17 and Th1. (Master's theses Theses and Dissertations Master). Al Akhawayn University, Morocco
https://search.emarefa.net/detail/BIM-647878
Modern Language Association (MLA)
Chehboun, Salma. In vitro production of interleukin 27 and Its immunoregulatory function upon CD4 differentiation into Th17 and Th1. (Master's theses Theses and Dissertations Master). Al Akhawayn University. (2010).
https://search.emarefa.net/detail/BIM-647878
American Medical Association (AMA)
Chehboun, Salma. (2010). In vitro production of interleukin 27 and Its immunoregulatory function upon CD4 differentiation into Th17 and Th1. (Master's theses Theses and Dissertations Master). Al Akhawayn University, Morocco
https://search.emarefa.net/detail/BIM-647878
Language
English
Data Type
Arab Theses
Record ID
BIM-647878
