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Genetic detection and pathological finding of BVDV and BHV-1 in camel calves
Other Title(s)
الاستكشاف الجيني و التغيرات الباثولوجية للإصابة بفيروسي الإسهال البقري الفيروسي و فيروس الهربس البقري في عجول الجمال
Joint Authors
Hassanin, Thana Kamal
al-Madbuli, Midhat Mustafa
Jafar, Jihan Abd Allah Muhammad
Salim, Halah Abd al-Rahim Ali
Source
Assiut Veterinary Medical Journal
Issue
Vol. 61, Issue 146 (31 Jul. 2015), pp.34-45, 12 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2015-07-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The objectives of this study are directed to employ multiplex PCR assay and some virological techniques for detection of bovine viral diarrhea virus (BVDV) and bovine herps virus-1 (BHV-1) in clinical samples with clarification of their pathological effects on camel calves.
A total of 124 different types of samples were collected from 43 camel calves (clinically ill n = 28, apparently healthy n = 10 and recently dead camel calves n = 5), from two different localities in Egypt.
All samples were subjected to virological (immunoflurece and immunoperoxidase) and molecular investigation.
Tissue samples from recently dead animals were subjected to histopathological studies.
BHV-1 was detected in 6(13.95 %) using immunoflurece versus to 7(16.27 %) by immunoperoxidase while BVDV was detected with same percentage (11.62 %) in both techniques.
Results of PCR demonstrated a specific amplicon of 389bp indicating the presence of BHV-1 in 8(21.21 %) out of examined 33 clinically ill animals.
While successful amplification of 288bp indicating the presence of BVDV in 6 (13.95 %) out of total 43 animals under investigation.
Histopathological examinations revealed encephalitis and necrosis in the brain with interstitial lymphocytic pneumonia.
Lymphocytic portal hepatitis, hyperplasia of spleen follicles, ulcerative enteritis and hemorrhagic lymphadenitis were observed.
Results concluded that the use of multiplex PCR can be a rapid cost-effective way for simultaneous detection so that consider a valuable practical diagnostic tool for BVDV and BHV-1 in clinical samples.
Immunoflurecent and immunoperoxidase regard as simple confirmative tools for identification of both viruses.
This study confirmed the lesions in several organs of camel calves infected with BVD and BHV-1.
American Psychological Association (APA)
Jafar, Jihan Abd Allah Muhammad& Hassanin, Thana Kamal& Salim, Halah Abd al-Rahim Ali& al-Madbuli, Midhat Mustafa. 2015. Genetic detection and pathological finding of BVDV and BHV-1 in camel calves. Assiut Veterinary Medical Journal،Vol. 61, no. 146, pp.34-45.
https://search.emarefa.net/detail/BIM-648249
Modern Language Association (MLA)
Jafar, Jihan Abd Allah Muhammad…[et al.]. Genetic detection and pathological finding of BVDV and BHV-1 in camel calves. Assiut Veterinary Medical Journal Vol. 61, no. 146 (Jul. 2015), pp.34-45.
https://search.emarefa.net/detail/BIM-648249
American Medical Association (AMA)
Jafar, Jihan Abd Allah Muhammad& Hassanin, Thana Kamal& Salim, Halah Abd al-Rahim Ali& al-Madbuli, Midhat Mustafa. Genetic detection and pathological finding of BVDV and BHV-1 in camel calves. Assiut Veterinary Medical Journal. 2015. Vol. 61, no. 146, pp.34-45.
https://search.emarefa.net/detail/BIM-648249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 43-45
Record ID
BIM-648249