In vitro induction of the acrosome reaction in local Egyptian rams spermatozoa by calcium ionosphere (a23187)‎

Abstract EN

The main objective of this work was to develop an efficient in vitro test (acrosome reaction, AR) to predict fertility in rams spermatozoa in the Egyptian local rams by using Ca++ionophore.

Semen was collected for 5 successive weeks from four Rahmani and four Ossimi Egyptian local rams.

Fresh collected semen was initially evaluated.

Calcium ionophore was used to stimulate the sperm to undergo acrosome reaction (AR).

Results showed that differences in all initial semen characteristic values were not significant among Rahmani rams.

The same results were obtained in Ossimi rams, except mass motility (88.75%, P<0.05).

After Induction of AR, results showed in Rahmani and Ossimi breeds that live-reacted spermatozoa% (LR) was the highest (34.25 and 34.50%, P<0.05) in ram No.

1 of both breeds when compared to other rams in both breeds, respectively.

Conception rate (CR) after natural mating revealed individual differences among rams within each breed, but the differences were significant (P<0.05) within Ossimi breed only.

Ram No.

1 of both breeds showed the highest CR compared to other rams of the same breed.

However, the mean of CR was insignificantly higher in Rahmani than Ossimi breed (89.19 vs.

81.08%).

There was positive correlation between percentage of live-reacted spermatozoa and CR in Rahmani breed (r=0.64, P<0.01) and in Ossimi breed (r=0.96, P<0.01).

In addition, there was a significant correlation between the percentage of mass motility and CR (r=0.70, P<0.01) in Ossimi breed.

It could be concluded that lamb rams fertility of local sheep can be predicted using acrosome reaction test with Ca++ionophore